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ZFPM1 Antibody

Images

 
Western Blot: ZFPM1 Antibody [NB100-68241] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-68241 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 ...read more
Immunohistochemistry-Paraffin: ZFPM1 Antibody [NB100-68241] - Human prostate carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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ZFPM1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 175 and 225 of human friend of GATA-1 (zinc finger protein, multi type 1) using the numbering given in entry NP_722520.2 (GeneID 161882).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZFPM1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZFPM1 Antibody

  • FOG-1
  • FOG1ZNF408
  • FOGfriend of GATA-1
  • Friend of GATA 1
  • Friend of GATA protein 1
  • ZFN89A
  • Zinc finger protein 89A
  • Zinc finger protein multitype 1
  • zinc finger protein ZFPM1
  • zinc finger protein, multitype 1
  • ZNF89A

Background

FOG1/ZFPM1 was identified in a yeast two-hybrid screen for GATA-1 interacting proteins. GATA-1 is zinc finger transcription factor whose expression is restricted to hematopoietic cells and plays and essential role in erythroid development. FOG1/ZFPM1 is highly expressed in erythroid cells and megakaryocytes and appears to play both a GATA-1-dependent and independent role in erythropoiesis and megakaryopoiesis. FOG1/ZFPM1 associates with GATA-1 as well as GATA-2 and GATA-3 as a heterodimer to activate or repress transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional ZFPM1 Products

Array NB100-68241

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Bioinformatics

Gene Symbol ZFPM1
Entrez
Uniprot