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![Western Blot: WHSC1 Antibody (CL1063) [NBP2-52963] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1063-Western-Blot-NBP2-52963-img0007.jpg "Western Blot: WHSC1 Antibody (CL1063) [NBP2-52963] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of U-251 cells using the Anti-WHSC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1063-Immunocytochemistry-Immunofluorescence-NBP2-52963-img0008.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human colorectal cancer shows strong nuclear immunoreactivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1063-Immunohistochemistry-Paraffin-NBP2-52963-img0002.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human cerebral cortex shows moderate positivity in neuronal cell nuclei.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1063-Immunohistochemistry-Paraffin-NBP2-52963-img0001.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1063-Immunohistochemistry-Paraffin-NBP2-52963-img0003.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human testis shows nuclear immunoreactivity in a subset of cells in seminiferous tubules.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1063-Immunohistochemistry-Paraffin-NBP2-52963-img0004.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human tonsil shows nuclear positivity in the reaction centrum cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1063-Immunohistochemistry-Paraffin-NBP2-52963-img0005.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human ventricular cancer shows strong nuclear immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1063-Immunohistochemistry-Paraffin-NBP2-52963-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: WHSC1 Antibody (CL1057) [NBP2-52962] - Staining of U-251 cells using the Anti-WHSC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1057-Immunocytochemistry-Immunofluorescence-NBP2-52962-img0007.jpg "Immunocytochemistry/Immunofluorescence: WHSC1 Antibody (CL1057) [NBP2-52962] - Staining of U-251 cells using the Anti-WHSC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1057) [NBP2-52962] - Staining of human colorectal cancer shows strong nuclear immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1057-Immunohistochemistry-Paraffin-NBP2-52962-img0002.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1057) [NBP2-52962] - Staining of human breast cancer shows moderate nuclear immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1057-Immunohistochemistry-Paraffin-NBP2-52962-img0001.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1057) [NBP2-52962] - Staining of human fallopian tube shows nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1057-Immunohistochemistry-Paraffin-NBP2-52962-img0003.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1057) [NBP2-52962] - Staining of human liver shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1057-Immunohistochemistry-Paraffin-NBP2-52962-img0004.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1057) [NBP2-52962] - Staining of human small intestine shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1057-Immunohistochemistry-Paraffin-NBP2-52962-img0005.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1057) [NBP2-52962] - Staining of human ventricular cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WHSC1-Antibody-CL1057-Immunohistochemistry-Paraffin-NBP2-52962-img0006.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining in human testis and liver tissues using anti-NSD2 antibody. Corresponding NSD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WHSC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82633-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining in human testis and liver tissues using anti-NSD2 antibody. Corresponding NSD2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: WHSC1 Antibody [NBP1-82633] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/WHSC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82633-img0004.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/WHSC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82633-img0009.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/WHSC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82633-img0010.jpg)
![Western Blot: WHSC1 Antibody [H00007468-B01P] - Analysis of WHSC1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/WHSC1-Antibody-Western-Blot-H00007468-B01P-img0005.jpg "Western Blot: WHSC1 Antibody [H00007468-B01P] - Analysis of WHSC1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: WHSC1 Antibody [H00007468-B01P] - Analysis of purified antibody to WHSC1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/WHSC1-Antibody-Immunocytochemistry-Immunofluorescence-H00007468-B01P-img0004.jpg)
![Western Blot: WHSC1 Antibody [H00007468-B01P] - Analysis of WHSC1 expression in transfected 293T cell line by WHSC1 polyclonal antibody. Lane1:WHSC1 transfected lysate(71.17 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/fullsize/WHSC1-Antibody-Western-Blot-H00007468-B01P-img0006.jpg)
![Immunohistochemistry: WHSC1 Antibody [NBP3-38487] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using WHSC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38487_rabbit-whsc1-pab-101220241224193.jpg "Immunohistochemistry: WHSC1 Antibody [NBP3-38487] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using WHSC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: WHSC1 Antibody [NBP3-38487] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using WHSC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38487_rabbit-whsc1-pab-101220241233826.jpg)
![Western Blot: WHSC1 Antibody [NBP3-38487] - Western blot analysis of various lysates using WHSC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38487_rabbit-whsc1-pab-10122024125280.jpg)
![Western Blot: WHSC1 Antibody (PSH02-39) [NBP3-32901] - Western blot analysis of WHSC1 on different lysates with Rabbit anti-WHSC1 antibody (NBP3-32901) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32901) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32901_rabbit-whsc1-mab-psh02-39-28820241246163.jpg "Western Blot: WHSC1 Antibody (PSH02-39) [NBP3-32901] - Western blot analysis of WHSC1 on different lysates with Rabbit anti-WHSC1 antibody (NBP3-32901) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32901) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: WHSC1 Antibody (PSH02-39) [NBP3-32901] - Immunohistochemical analysis of paraffin-embedded Rat testis tissue with Rabbit anti-WHSC1 antibody (NBP3-32901) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32901_rabbit-whsc1-mab-psh02-39-298202414262639.jpg)
![Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-30910] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/WHSC1-Antibody-Immunohistochemistry-Paraffin-NBP1-30910-img0003.jpg "Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-30910] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.")
![SDS-Page: Recombinant Human WHSC1 Protein [H00007468-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007468-P01-1.jpg "SDS-Page: Recombinant Human WHSC1 Protein [H00007468-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")