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Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

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ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Standard Reference Curve
ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions.
ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Lysate, Tissue
Standard Curve Range
78.125 - 5000 pg/mL
Sensitivity
50 pg/mL

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Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) Summary

Standard Curve Range
78.125 - 5000 pg/mL
Sensitivity
50 pg/mL
Assay Type
Competitive ELISA
Spike Recovery
95.5%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
VPS35

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. 100X Poly HRP Concentrate - 150uL
  2. 10X Wash Buffer Concentrate - 100mL
  3. 5X VPS35 Extraction Reagent - 10mL
  4. Anti-VPS35 Biotinylated Antibody Concentrate - 150uL
  5. Anti-VPS35 Biotinylated Antibody Diluent (Green) - 13mL
  6. Anti-VPS35 Immunoassay Plate - 1 plate
  7. Recombinant VPS35 Standard - 2 vials
  8. Standard and Sample Diluent (Red) - 50mL
  9. Stop Solution - 13mL
  10. Streptavidin: HRP Diluent (Purple) - 13mL
  11. TMB Substrate - 13mL

Alternate Names for Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric)

  • DKFZp434P1672
  • FLJ10752
  • FLJ13588
  • FLJ20388
  • hVPS35
  • Maternal-embryonic 3
  • MEM3DKFZp434E1211
  • vacuolar protein sorting 35 (yeast homolog)
  • vacuolar protein sorting 35 (yeast)
  • vacuolar protein sorting 35 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 35
  • Vesicle protein sorting 35

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol VPS35