VPS35 Antibody

Chemokine receptor D6 expression has been documented in human skin, small intestine, large intestine, appendix, lymph node, vessels, tonsil, spleen, as well as from rat brain tissues. ESTs have been isolated from cancerous human lung, uterus, cervix, placenta, blood, and from normal human placenta libraries. Caution: G protein-coupled receptor D6 was previously called CCR10 and/or CCR9. CCR9 and C-C chemokine receptor 9 referred to both chemokine receptor D6 and the unrelated receptor GPR-9-6.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-P, WB.

Showing Publications 1 - 10 of 30. Show All 30 Publications. Collapse Publications.

Publications using NB100-1397	Applications	Species
Kramer BA, Pelkmans L Cellular state determines the multimodal signaling response of single cells bioRxiv 2019-12-19 (ICC/IF, Human)	ICC/IF	Human
Tarique Bagalkot, Alexander Sorkin Amphetamine Induces Sex-Dependent Loss of the Striatal Dopamine Transporter in Sensitized Mice eNeuro 2023-12-19 [PMID: 38164591]
Yang Z, Feng Z, Li Z, Teasdale R Multifaceted Roles of Retromer in EGFR Trafficking and Signaling Activation Cells 2022-10-25 [PMID: 36359754] (WB, Human)	WB	Human
Huang Y, Huang H, Zhou L et al. Mutant D620N and VPS35 induces motor dysfunction and impairs DAT-mediated dopamine recycling pathway Human molecular genetics 2022-06-29 [PMID: 35766879]
Chen KE, Guo Q, Hill TA et al. De novo macrocyclic peptides for inhibiting, stabilizing, and probing the function of the retromer endosomal trafficking complex Science Advances 2021-12-03 [PMID: 34851660]
Cui Y, Yang Z, Flores-Rodriguez N et al. Formation of retromer transport carriers is disrupted by the Parkinson disease-linked Vps35 D620N variant Traffic (Copenhagen, Denmark) 2020-12-21 [PMID: 33347683]
Han J, Goldstein LA, Hou W et al. Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport Autophagy 2020-05-25 [PMID: 32397873] (WB, Mouse)	WB	Mouse
Yasa S, Modica G, Sauvageau E Et Al. CLN3 regulates endosomal function by modulating Rab7A effector interactions J. Cell. Sci. 2020-02-07 [PMID: 32034082] (WB, Human)	WB	Human
JulliE D, Stoeber M, Sibarita JB, et al. A Discrete Presynaptic Vesicle Cycle for Neuromodulator Receptors Neuron 2019-12-05 [PMID: 31837915]
Pan, X;Meriin, A;Huang, G;Kandror, KV; Insulin-responsive amino peptidase follows the Glut4 pathway but is dispensable for the formation and translocation of insulin-responsive vesicles Mol. Biol. Cell 2019-06-01 [PMID: 30943117] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Cui Y, Carosi JM, Yang Z et al. Retromer has a selective function in cargo sorting via endosome transport carriers J. Cell Biol. 2018-12-17 [PMID: 30559172] (ICC/IF, Human)	ICC/IF	Human
Tammineni P, Jeong Y Y et al. Impaired axonal retrograde trafficking of the retromer complex augments lysosomal deficits in Alzheimer's disease neurons. Hum Mol Genet 2017-11-15 [PMID: 28973312] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Wu S, Fagan RR, Uttamapinant C et al. The Dopamine Transporter Recycles via a Retromer-Dependent Postendocytic Mechanism: Tracking Studies Using a Novel Fluorophore-Coupling Approach J Neurosci. 2017-09-26 [PMID: 28847807] (IHC-P, Mouse)	IHC-P	Mouse
Lobingier BT, Huttenhain R, Eichel K et al. An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Cell. 2017-04-06 [PMID: 28388416]
Elwell CA, Czudnochowski N, von Dollen J et al. Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction. Elife. 2017-03-02 [PMID: 28252385] (WB, Mouse)	WB	Mouse
Paul B, Kim HS, Kerr MC et al. Structural basis for the hijacking of endosomal sorting nexin proteins by Chlamydia trachomatis. Elife. 2017-02-22 [PMID: 28226239] (Human)		Human
Yin J, Liu X, He Q et al. Vps35-dependent recycling of Trem2 regulates microglial function. Traffic 2016-09-26 [PMID: 27717139]
Yang Z, Hong LK, Follett J et al. Functional characterization of retromer in GLUT4 storage vesicle formation and adipocyte differentiation. FASEB J. 2015-11-18 [PMID: 26581601] (ICC/IF, WB, Human, Mouse)	ICC/IF, WB	Human, Mouse
Yin P, Hong Z, Yang X et al. A role for retromer in hepatitis C virus replication. Cell. Mol. Life Sci. 2015-08-23 [PMID: 26298293] (ICC/IF, Human) Details: VPS35 antibody (Imgenex: IMG-3575) was used for ICC-IF analysis of Huh 7.5.1 cells, JFH1-plasmid infected Huh 7.5.1 cells, OR6 cells (which harbors the full-length genotype 1b HCV RNA) and Cured OR6 cells (OR6 cells treated with 10 IU/ml of IFN alpha for 7 days) [Fig 2A/B, 3A/B].	ICC/IF	Human
Hsiao JC, Chu LW, Lo YT et al. Intracellular Transport of Vaccinia Virus in HeLa Cells Requires WASH-VPEF/FAM21-Retromer Complexes and Recycling molecules Rab11 and Rab22 J. Virol. 2015-06-03 [PMID: 26041286] (ICC/IF, WB, Human) Details: VPS35 antibody was used for ICC-IF on HeLa cells that were transfected with a plasmid expressing mock/GFP or GFP-VPS26. The antibody was also used for WB application on lysates of HeLa cells which were either mock transfected /Control or transfected with siRNA duplexes targeting CypB (Si-CypB), SNX1 (Si-SNX1), WASH (Si-WASH), or Vps35 (Si-Vps35) [FIG 6].	ICC/IF, WB	Human
Fuse A, Furuya N, Kakuta S et al. VPS29-VPS35 intermediate of retromer is stable and may be involved in the retromer complex assembly process. FEBS Lett. 2015-06-04 [PMID: 25937119] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Meisenberg C, Gilbert DC, Chalmers A et al. Clinical and cellular roles for TDP1 and TOP1 in modulating colorectal cancer response to irinotecan. Mol. Cancer Ther. 2014-12-18 [PMID: 25522766] (ICC/IF, Human)	ICC/IF	Human
Tabuchi M, Yanatori I, Kawai Y, Kishi F. Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron transporter DMT1. J Cell Sci. 2010-03-01 [PMID: 20164305] (WB) Details: Product cited: VPS35/MEM3 (IMG-3575) in HEp-2 epithelial cells transfected with various DMT1-II plasmids: WB: Fig 4A, cells treated with VPS35 siRNA or left untreated IF: Figs 3Ac, 3Bc, S1ac, S1bc, S1cc Note: The specificity of the IMG-3575 antibody was V	WB
Hong Z, Yang Y, Zhang C et al. The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport. Cell Res. 2009-12-01 [PMID: 19935774] (WB) Details: VPS35/MEM3 (IMG-3575) cited: WB (HeLa), Fig 2C.	WB
Scott KL, Kabbarah O, Liang MC et al. GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Nature 2009-06-01 [PMID: 19553991]
Rodriguez LZ The regulation of VPS34 and WNK1 kinase in the proximal tubule of the kidney in health and disease Thesis 2023-01-01 (WB, IHC, Mouse)	WB, IHC	Mouse
Rodriguez LZ The regulation of VPS34 and WNK1 kinase in the proximal tubule of the kidney in health and disease Thesis 2023-01-01 (WB, IHC, Mouse)	WB, IHC	Mouse
Rodriguez LZ The regulation of VPS34 and WNK1 kinase in the proximal tubule of the kidney in health and disease Thesis 2023-01-01 (WB, IHC, Mouse)	WB, IHC	Mouse
Sullivan CP, Jay AG, Stack EC et al. Retromer disruption promotes amyloidogenic APP processing. Neurobiol Dis. 2011-08-01 [PMID: 21515373] (WB, ICC/IF) Details: VPS35/MEM3 (IMG-3575). WB: HEk293 cell line transfected with Vps35 siRNA, Fig 1A. IF/ICC: HEk293 cell line transfected with Vps35 siRNA, Fig 2B.Note: The Vps35 (IMG-3575) antibody was siRNA validated by WB & IF/ICC in HEk293 cells, Fig 1A & 1B.	WB, ICC/IF
Zhang P, Yu L, Gao J et al. Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21. Genomics 2000-12-01 [PMID: 11112353]
Show All 30 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: jerome cattin	IF	Rat	10/29/2020	View Summary Application Immunofluorescence Sample Tested INS-1 Species Rat Lot C3 E021219
Enlarge	4 reviewed by: jerome cattin	ICC	Human	05/28/2020	View Summary Application Immunocytochemistry Sample Tested hela cell Species Human Lot C3 E021219
Enlarge	5 reviewed by: Verified Customer	WB	Human	05/28/2020	View Summary Application Western Blot Sample Tested HEK293T cell lysate Species Human Lot C3 E0212129
Enlarge	5 reviewed by: Verified Customer	WB	Canine, Chicken, Human	01/07/2020	View Summary Application Western Blot Sample Tested DF-1,MDCK canine kidney epithelial cell line,RPE-1,SH-SY5Y whole cell lysates Species Canine, Chicken, Human Lot C3E230818 Comments Comments samples prepared in 1.5x lammeli buffer, 10ug of protein loaded per lane transferred onto nitrocellulose at 100V for 1.5 hours 4 degrees. blotting coditions membrane blocked in 5% milk in PBS 0.1% tween 20 (PBST) for 1 hour primary ab incubated 1:2000 in 5% milk in PBS at 4 degrees for 20 hours washed 3X with PBST secondary donkey anti goat HPR 1:5000 for 1 hour room temp washed 2x PBST, 1X PBS blot developed using ECL prime reagent (GE healthcare )
Enlarge	5 reviewed by: Verified Customer	ICC	Human	01/07/2020	View Summary Application Immunocytochemistry Sample Tested RPE-1 cells,RPE-1 Species Human Lot C3E230818

Array NB100-1397

• VPS35 Antibodies
• VPS35 ELISA Kit
• VPS35 Lysate
• VPS35 Proteins