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VP26B Antibody

Images

 
Immunohistochemistry-Paraffin: VP26B Antibody [NBP1-92575] - Staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: VP26B Antibody [NBP1-92575] - Staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer.
Immunohistochemistry-Paraffin: VP26B Antibody [NBP1-92575] - Staining of human placenta shows weak cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: VP26B Antibody [NBP1-92575] - Staining of human prostate shows weak membranous positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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VP26B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RRYFKQQEVVLWRKGDIVRKSMSHQAAIASQRFEGTTSLGEVRTPSQLSDNNCRQ
Predicted Species
Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS26B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot Reported in scientific literature (PMID:35045281)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
VP26B Recombinant Protein Antigen (NBP1-92575PEP)
Publications
Read Publications using
NBP1-92575 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 35045281).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VP26B Antibody

  • MGC10485
  • Pep8b
  • vacuolar protein sorting 26 homolog B (S. pombe)
  • vacuolar protein sorting 26 homolog B (yeast)
  • vacuolar protein sorting-associated protein 26B
  • Vesicle protein sorting 26B

Background

Probable component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2Rand M6PR) from endosomes to the trans-Golgi network

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC

Publications for VP26B Antibody (NBP1-92575)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC-FrFl, IP, KO, WB.


Filter By Application
ICC/IF
(1)
IHC-FrFl
(1)
IP
(1)
KO
(1)
WB
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP1-92575 Applications Species
Mishra S, Knupp A, Kinoshita C et al. Pharmacologic enhancement of retromer rescues endosomal pathology induced by defects in the Alzheimer's gene SORL1 Stem cell reports 2023-10-26 [PMID: 37949073] (WB, Human) WB Human
Qureshi YH, Berman DE, Klein RL, Patel VM et al. Retromer repletion with AAV9-VPS35 restores endosomal function in the mouse hippocampus bioRxiv 2019-05-15 (WB, Mouse) WB Mouse
Christensen S, Narimatsu Y, Simoes S et al. Endosomal trafficking is required for glycosylation and normal maturation of the Alzheimers-associated protein sorLA bioRxiv 2020-07-14 (WB, KO, Mouse) WB, KO Mouse
Qureshi YH, Berman DE, Marsh SE et al. The neuronal retromer can regulate both neuronal and microglial phenotypes of Alzheimer's disease Cell reports 2022-01-18 [PMID: 35045281] (WB, Mouse) WB Mouse
Simoes S, Guo J, Buitrago L et al. Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling Cell reports 2021-12-28 [PMID: 34965419] (IHC-FrFl, IP, ICC/IF, WB, Human, Mouse) IHC-FrFl, IP, ICC/IF, WB Human, Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS26B