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TBC1D5 Antibody

Images

 
Independent Antibodies: Western Blot: TBC1D5 Antibody [NBP2-38237] - Analysis using Anti-TBC1D5 antibody NBP2-38237 (A) shows similar pattern to independent antibody NBP1-93653 (B).
Immunocytochemistry/ Immunofluorescence: TBC1D5 Antibody [NBP2-38237] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TBC1D5 Antibody [NBP2-38237] - Staining of human fallopian tube shows strong positivity in glandular cells.
Western Blot: TBC1D5 Antibody [NBP2-38237] - MEF: mouse embryonic feeder cell lysate, B16-F10: mouse melanoma cell lysate. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: TBC1D5 Antibody [NBP2-38237] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemistry-Paraffin: TBC1D5 Antibody [NBP2-38237] - Staining of human lymph node shows strong positivity in lymphocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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TBC1D5 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SSSPSVESLPGGREFTGSPPSSATKKDSFFSNISRSRSHSKTMGRKESEEELEAQISFLQGQLNDLDAMCKYCAKVMDTHLVNIQDVILQEN
Predicted Species
Mouse (95%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBC1D5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. TBC1D5 antibody validated for WB from a verified customer review.
Control Peptide
TBC1D5 Protein (NBP2-38237PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-38237 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TBC1D5 Antibody

  • TBC1 domain family, member 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC

Publications for TBC1D5 Antibody (NBP2-38237) (0)

There are no publications for TBC1D5 Antibody (NBP2-38237).
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Review for TBC1D5 Antibody (NBP2-38237) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-38237:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot TBC1D5 NBP2-38237
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 07/18/2018
View

Summary

ApplicationWestern Blot
Sample TestedB16-F10 mouse melanoma cell line,MEF mouse embryonic feeder cells
SpeciesMouse
LotR33313

Comments

CommentsTotal cell lysates from MEF and B16-10 were subjected to western blot. PVDF membrane were probed with 1 um/ml TBC1D5 Antibody (NBP2-38237). A specific band was detected for RAB5C at approximately 89 kDa. This experiment was conducted under reducing conditions

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TBC1D5 Antibody (NBP2-38237) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

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Recent Reviews

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5
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1
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Verified Customer
07/18/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TBC1D5
Uniprot