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Properdin Antibody

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Western blot shows lysates of human serum, mouse serum, and rat serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Properdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8216) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Properdin at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human serum, mouse serum, and rat serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Properdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8216) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF8216-SP
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Properdin Antibody Summary

Immunogen
Human embryonic kidney cell line HEK293-derived human Properdin
Asp28-Leu469
Accession # P27918
Specificity
Detects human, mouse, and rat Properdin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CFP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF8216 in the following application:
Publications
Read Publication using
AF8216 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Properdin Antibody

  • BFD
  • CFP
  • Complement Factor P
  • complement factor properdin
  • PFC
  • PFCcomplement
  • PFD
  • Properdin

Background

Human Properdin, also known as Complement factor P (CFP), is a 53 kDa secreted  glycoprotein that exists in plasma as dimers, trimers or tetramers. It a positive regulator of the alternate pathway of complement.  Properdin  binds to and stabilizes the C3- and C5-convertase enzyme complexes and is thought to connect humoral and cellular immunity. Properdin released from activated neutrophils may play a major role in pattern recognition, whereas plasma properdin may primarily stabilize convertase. Properdin deficiency results in higher susceptibility to bacterial infections. Full-length human Properdin shares 77 % sequence identity with mouse Properdin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Properdin Antibody
AF8216
Properdin Antibody
Species: Hu, Mu, Rt
Applications: WB

Publications for Properdin Antibody (AF8216)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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ELISA Development
(1)
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Review for Properdin Antibody (AF8216) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF8216:
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IF
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Properdin AF8216
Enlarge 		5
reviewed by:
Jing Zhu
 IF Mouse 11/27/2018
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Summary

ApplicationImmunofluorescence
Sample TestedMouse kidney
SpeciesMouse
LotCILL011811A

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Recent Reviews

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1
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Immunofluorescence Properdin AF8216
Enlarge
 Jing Zhu
11/27/2018
Application: IF
Species: Mouse
read this review

Bioinformatics

Gene Symbol CFP
Uniprot