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Vitronectin Antibody (347317) [Unconjugated]

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Vitronectin was detected in immersion fixed frozen sections of fetal mouse brain using 10 µg/mL Rat Anti-Mouse Vitronectin Monoclonal Antibody (Catalog # MAB38751) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Vitronectin was detected in immersion fixed frozen sections of fetal mouse brain using 10 µg/mL Rat Anti-Mouse Vitronectin Monoclonal Antibody (Catalog # MAB38751) overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC
Clone
347317
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB38751-SP
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Vitronectin Antibody (347317) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Vitronectin
Asp20-Lys478
Accession # AAH12690
Specificity
Detects mouse Vitronectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Vitronectin is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
VTN
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB38751 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vitronectin Antibody (347317) [Unconjugated]

  • Complement S-protein
  • epibolin
  • Serum Spreading Factor
  • Serum-spreading factor
  • Somatomedin B
  • S-protein
  • V75
  • vitronectin (serum spreading factor, somatomedin B, complement S-protein)
  • Vitronectin
  • VN
  • VNT
  • VTN

Background

Vitronectin is a 71 kDa secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably alpha v beta 3) and thrombin. The 459 amino acid (aa) mature mouse Vitronectin shows 74% aa sequence identity with human Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65 kDa and 10 kDa components.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Vitronectin Antibody (MAB38751)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Bioassay, Flow Cytometry, IHC, IHC-Fr.


Filter By Application
Bioassay
(1)
Flow Cytometry
(1)
IHC
(3)
IHC-Fr
(2)
All Applications
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Mouse
(8)
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Showing Publications 1 - 8 of 8.
Publications using MAB38751 Applications Species
C Yin, M Kato, T Tomita, Y Han, S Hiratsuka Suppression of CEBPdelta recovers exhaustion in anti-metastatic immune cells Scientific Reports, 2023-03-08;13(1):3903. 2023-03-08 [PMID: 36890150] (Mouse) Mouse
S Grubb, C Cai, BO Hald, L Khennouf, RP Murmu, AGK Jensen, J Fordsmann, S Zambach, M Lauritzen Precapillary sphincters maintain perfusion in the cerebral cortex Nat Commun, 2020-01-20;11(1):395. 2020-01-20 [PMID: 31959752] (IHC, Mouse) IHC Mouse
Z Bagi, Y Couch, Z Broskova, F Perez-Bald, T Yeo, S Davis, R Fischer, NR Sibson, BG Davis, DC Anthony Extracellular vesicle integrins act as a nexus for platelet adhesion in cerebral microvessels Sci Rep, 2019-11-01;9(1):15847. 2019-11-01 [PMID: 31676801] (Flow Cytometry, Mouse) Flow Cytometry Mouse
S Hiratsuka, T Tomita, T Mishima, Y Matsunaga, T Omori, S Ishibashi, S Yamaguchi, T Hosogane, H Watarai, M Omori-Miya, T Yamamoto, N Shibata, A Watanabe, H Aburatani, M Tomura, KA High, Y Maru Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the�lung EMBO Mol Med, 2018-07-01;10(7):. 2018-07-01 [PMID: 29930175] (Bioassay, Mouse) Bioassay Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse) IHC Mouse
Wang X, Rodda L, Bannard O, Cyster J Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. J Immunol, 2014-04-16;192(10):4601-9. 2014-04-16 [PMID: 24740506] (IHC, Mouse) IHC Mouse
Franco M, Roswall P, Cortez E, Hanahan D, Pietras K Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression. Blood, 2011-07-21;118(10):2906-17. 2011-07-21 [PMID: 21778339] (IHC-Fr, Mouse) IHC-Fr Mouse
Zhao Z, Chen Y, Wang J Age-related retinopathy in NRF2-deficient mice. PLoS ONE, 2011-04-29;6(4):e19456. 2011-04-29 [PMID: 21559389] (IHC-Fr, Mouse) IHC-Fr Mouse

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Bioinformatics

Gene Symbol VTN
Uniprot