![Western Blot: VAP-A Antibody [NBP1-31237] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Western-Blot-NBP1-31237-img0013.jpg "Western Blot: VAP-A Antibody [NBP1-31237] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, Pm, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VAP-A. The exact sequence is proprietary. |
| Localization | Cell membrane, Single-pass type IV membrane protein, Endomembrane system, Single-pass type IV membrane protein | |
| Predicted Species | Bovine (100%), Chimpanzee (100%), Zebrafish (100%), Chicken (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | VAPA |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH7), 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for VAP-A Antibody (NBP1-31237)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | VAPA |
![Immunocytochemistry/Immunofluorescence: VAP-A Antibody [NBP1-31237] - WDR44 tubules are in close contact with the ER via binding to VAPA/B. Confocal images of transfected HeLa cells showing colocalization of GRAF2-RFP tubules with GFP-VAPA. Image collected and cropped by CiteAb from the following publication (//rupress.org/jcb/article/doi/10.1083/jcb.201811014/151714/GRAF2-WDR44-and-MICAL1-mediate-Rab81011dependent) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31237-img0014.jpg "Immunocytochemistry/Immunofluorescence: VAP-A Antibody [NBP1-31237] - WDR44 tubules are in close contact with the ER via binding to VAPA/B. Confocal images of transfected HeLa cells showing colocalization of GRAF2-RFP tubules with GFP-VAPA. Image collected and cropped by CiteAb from the following publication (//rupress.org/jcb/article/doi/10.1083/jcb.201811014/151714/GRAF2-WDR44-and-MICAL1-mediate-Rab81011dependent) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human breast cancer, using VAPA antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Immunohistochemistry-Paraffin-NBP1-31237-img0007.jpg "Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human breast cancer, using VAPA antibody at 1:500 dilution.")
![Western Blot: VAP-A Antibody [NBP1-31237] - Analysis of antibody on A:NIH-3T3 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/VAP-A-Antibody-Western-Blot-NBP1-31237-img0008.jpg "Western Blot: VAP-A Antibody [NBP1-31237] - Analysis of antibody on A:NIH-3T3 12% SDS PAGE diluted at 1:1000")
![Western Blot: VAP-A Antibody [NBP1-31237] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Western-Blot-NBP1-31237-img0011.jpg "Western Blot: VAP-A Antibody [NBP1-31237] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human lung adenocarcinoma, using VAPA antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Immunohistochemistry-Paraffin-NBP1-31237-img0006.jpg "Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human lung adenocarcinoma, using VAPA antibody at 1:500 dilution.")
![Western Blot: VAP-A Antibody [NBP1-31237] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAP-A antibody [N1N3] (NBP1-31237) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31237_rabbit-polyclonal-vap-a-antibody-91020242001081.jpg "Western Blot: VAP-A Antibody [NBP1-31237] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAP-A antibody [N1N3] (NBP1-31237) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")