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VAMP-8 Antibody (JF0963)

Images

 
Western Blot: VAMP-8 Antibody (JF0963) [NBP2-66777] - Western blot analysis of VAMP-8 on U93 cell lysates using anti-VAMP-8 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: VAMP-8 Antibody (JF0963) [NBP2-66777] - Staining VAMP8 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: VAMP-8 Antibody (JF0963) [NBP2-66777] - Analysis of paraffin-embedded mouse colon tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
Flow Cytometry: VAMP-8 Antibody (JF0963) [NBP2-66777] - Analysis of Hela cells with VAMP8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Western Blot: VAMP-8 Antibody (JF0963) [NBP2-66777] - Analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse kidney
Immunocytochemistry/ Immunofluorescence: VAMP-8 Antibody (JF0963) [NBP2-66777] - Staining VAMP8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: VAMP-8 Antibody (JF0963) [NBP2-66777] - Staining VAMP8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: VAMP-8 Antibody (JF0963) [NBP2-66777] - Analysis of paraffin-embedded human kidney tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: VAMP-8 Antibody (JF0963) [NBP2-66777] - Analysis of paraffin-embedded mouse brain tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
JF0963
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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VAMP-8 Antibody (JF0963) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human VAMP-8 aa1-29 / 100. (SwissProt: Q9BV40 Human; SwissProt: O70404 Mouse; SwissProt: Q9WUF4 Rat)
Localization
Lysosome membrane, Early endosome membrane, Late endosome membrane, Cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
VAMP8
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for VAMP-8 Antibody (JF0963)

  • EDB
  • EDBVAMP-8
  • Endobrevin
  • VAMP8
  • VAMP-8
  • vesicle-associated membrane protein 8 (endobrevin)
  • vesicle-associated membrane protein 8

Background

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VAMP-8 Antibody (NBP2-66777) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VAMP8