Novus Biologicals products are now on bio-techne.com

Syntaxin 7 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Analysis in human lymph node and pancreas tissues. Corresponding Syntaxin 7 RNA-seq data are presented for the same tissues.
Western Blot: Syntaxin 7 Antibody [NBP1-87497] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody [NBP1-87497] - Staining of human cell line U-251 MG shows localization to lysosomes.
Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.
Western Blot: Syntaxin 7 Antibody [NBP1-87497] - Analysis in human cell line Daudi.
Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Staining of human lymph node shows strong membranous positivity in germinal center cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Syntaxin 7 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STX7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. Syntaxin 7 antibody validated for WB from a verified customer review.
Control Peptide
Syntaxin 7 Protein (NBP1-87497PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-87497 in the following applications:

Publications
Read Publications using
NBP1-87497 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Syntaxin 7 Antibody

  • STX7
  • Syntaxin 7
  • syntaxin-7

Background

Syntaxin 7 is involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. Syntaxin 7 is found in late endosomes and lysosomes; whereas Syntaxin 12 is localized to the recycling endosome compartment, both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-33414
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF5448
Species: Hu, Mu, Rt
Applications: IHC, WB
AF5354
Species: Hu
Applications: IHC, WB
MAB6117
Species: Hu
Applications: IHC, WB
NBP1-87035
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-15706
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-00795
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-86984
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-45413
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, WB
H00010228-M01
Species: Hu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NB300-510
Species: Ca, Hu, Mu, Rt
Applications: ICC/IF, WB
NB110-74682
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-82785
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-36568
Species: Hu, Mu
Applications: Flow-IC, ICC/IF, IHC, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
MAB6617
Species: Hu
Applications: WB
NBP1-87497
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for Syntaxin 7 Antibody (NBP1-87497)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


Filter By Application
IF/IHC
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for Syntaxin 7 Antibody (NBP1-87497) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-87497:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Syntaxin 7 NBP1-87497
Enlarge
5
reviewed by:
Scott Messenger
WB Human 04/25/2017
View

Summary

ApplicationWestern Blot
Sample TestedT84 human colon carcinoma cell line
SpeciesHuman

Comments

Comments100 ug whole cell lysate, 15% gel

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Syntaxin 7 Antibody (NBP1-87497) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Syntaxin 7 Products

Research Areas for Syntaxin 7 Antibody (NBP1-87497)

Find related products by research area.

Blogs on Syntaxin 7

There are no specific blogs for Syntaxin 7, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Scott Messenger
04/25/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol STX7