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V-type proton ATPase subunit F Antibody

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Western Blot: V-type proton ATPase subunit F Antibody [NBP2-38942] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-38942] - Staining in human cerebral cortex and skeletal muscle tissues using anti-ATP6V1F antibody. ...read more
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-38942] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-38942] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-38942] - Staining of human skeletal muscle shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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V-type proton ATPase subunit F Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAKDSILRRARGMFTAEDL
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1F
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
V-type proton ATPase subunit F Protein (NBP2-38942PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for V-type proton ATPase subunit F Antibody

  • ATP6S14adenosinetriphosphatase 14k chain
  • ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
  • H(+)-transporting two-sector ATPase, 14kD subunit
  • MGC117321
  • MGC126037
  • MGC126038
  • vacuolar ATP synthase subunit F
  • vacuolar proton pump F subunit
  • Vacuolar proton pump subunit F
  • VATFATPase, vacuolar, 14 kD
  • V-ATPase 14 kDa subunit
  • V-ATPase F subunit
  • V-ATPase subunit F
  • Vma7
  • V-type proton ATPase subunit F

Background

V-type proton ATPase subunit F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATP6V1F
Uniprot