Home » UCH-L1/PGP9.5 » UCH-L1/PGP9.5 Antibodies » UCH-L1/PGP9.5 Antibody (671108) [Unconjugated]
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UCH-L1/PGP9.5 Antibody (671108) [Unconjugated]

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Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCH-L1/PGP9.5 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007) followed by HRP-conjugated Anti-Mouse IgG ...read more
UCH-L1/PGP9.5 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007) at 15 µg/mL overnight at 4 °C. Before incubation ...read more
Simple Western lane view shows lysates of human brain (cortex) tissue and mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected forUCH-L1/PGP9.5 at approximately 32 kDa (as indicated) using 10 ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP, KO
Clone
671108
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6007-SP
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UCH-L1/PGP9.5 Antibody (671108) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human UCH-L1/PGP9.5
Gln2-Ala223
Accession # P09936
Specificity
Detects human UCH-L1/PGP9.5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human UCH-L3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB6007 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UCH-L1/PGP9.5 Antibody (671108) [Unconjugated]

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

UCH-L1 (ubiquitin carboxyterminal hydrolase isozyme 1; also PGP9.5) is a 24-27 kDa member of the peptidase C12 family of enzymes. It shows restricted expression, being found in neurons and oocytes. UCH-L1 has dual enzymatic activity. As a monomer, it is a ubiquitin hydrolase that removes ubiquitin from modified proteins; as a homodimer, it acts as a ligase that creates ubiquitin dimers. In neurons, UCH-L1’s most important role appears to be that of generating free ubiquitin. Human UCH-L1 is 223 amino acids (aa) in length. It is O-glycosylated, ubiquitinated, and farnesylated; when farnesylated, it becomes associated with cell membranes. Three potential splice forms are reported. One shows a two aa substitution for aa 12-15, a second contains an alternative start site at Met82, and a third shows the same start site coupled with a deletion of aa 138-153. Full-length human UCH-L1 shares 95% aa identity with mouse UCH-L1.       

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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UCH-L1/PGP9.5
Antibody (671108) [Un ...
MAB6007
UCH-L1/PGP9.5 Antibody (671108) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC, IP, KO

Publications for UCH-L1/PGP9.5 Antibody (MAB6007)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IHC, IP, Surface Plasmon Resonance (SPR, Western Blot.


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IHC
(1)
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(1)
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(1)
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(3)
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Bioinformatics

Gene Symbol UCHL1
Uniprot