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UBE2J1 Antibody

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Western Blot: UBE2J1 Antibody [NBP1-85654] - Analysis in control (vector only transfected HEK293T lysate) and UBE2J1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654] - Analysis in human lymph node and skeletal muscle tissues using NBP1-85654 antibody. Corresponding UBE2J1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654] - Staining of human skeletal muscle shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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UBE2J1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2J1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
UBE2J1 Protein (NBP1-85654PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UBE2J1 Antibody

  • CGI-76
  • EC 6.3.2.19
  • HSPC153
  • HSPC205
  • HSU93243
  • HsUBC6e
  • MGC12555
  • NCUBE1
  • NCUBE-1
  • NCUBE1HSUBC6e
  • Non-canonical ubiquitin-conjugating enzyme 1
  • non-canonical ubquitin conjugating enzyme 1
  • UBC6e
  • Ubc6p
  • UBE2J1
  • ubiquitin-conjugating enzyme E2 J1
  • ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)
  • Yeast ubiquitin-conjugating enzyme UBC6 homolog E

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UBE2J1