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UBE2G1 Antibody

Images

 
Western Blot: UBE2G1 Antibody [NBP2-13500] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: UBE2G1 Antibody [NBP2-13500] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: UBE2G1 Antibody [NBP2-13500] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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UBE2G1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVA
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2G1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
UBE2G1 Protein (NBP2-13500PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UBE2G1 Antibody

  • E217K
  • EC 6.3.2.19
  • UBC7
  • UBE2G1
  • UBE2GE217K
  • Ubiquitin carrier protein G1
  • ubiquitin-conjugating enzyme E2 G1
  • ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7)
  • ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans)
  • ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast)
  • Ubiquitin-protein ligase G1

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE2G1