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Recombinant Human UbcH5a/UBE2D1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
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Support & Research

Recombinant Human UbcH5a/UBE2D1 Protein, CF Summary

Details of Functionality
Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1‑1 μM.
Source
E. coli-derived human UbcH5a/UBE2D1 protein
Met1-Met147
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2D1

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E2-616 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UbcH5a/UBE2D1 Protein, CF

  • E2(17)KB1
  • Stimulator of Fe transportUBC4/5 homolog
  • UBC4/5
  • UBC5A
  • UbcH5
  • UbcH5a
  • UBCH5AEC 6.3.2.19
  • UBCH5SFT
  • UBE2D1
  • Ubiquitin carrier protein D1
  • ubiquitin-conjugating enzyme E2 D1
  • Ubiquitin-conjugating enzyme E2(17)KB 1
  • Ubiquitin-conjugating enzyme E2-17 kDa 1
  • ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D1

Background

Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).

  1. Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
  2. Gehrke, S. et al. (2003) Blood 101:3288.
  3. Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
  4. Lorick, K. et al. (2005) Methods Enzymol. 398:54.
  5. Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
  6. Dynek, J. et al. (2010) EMBO J. 29:4128.
  7. Vanni, E. et al. (2012) J. Virol. 86:6323.

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Bioinformatics

Gene Symbol UBE2D1
Uniprot