Reactivity | MuSpecies Glossary |
Applications | WB, ELISA(Det) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Biotin |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant mouse TWEAK R/TNFRSF12 Glu28-Trp79 Accession # Q9CR75 |
Specificity | Detects mouse TWEAK R/TNFRSF12 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross‑reactivity with recombinant human TWEAK R is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | TNFRSF12A |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA Capture: Mouse TWEAK R/TNFRSF12 Antibody (Catalog # AF1610) ELISA Detection: Mouse TWEAK R/TNFRSF12 Biotinylated Antibody (Catalog # BAF1610) Standard: Recombinant Mouse TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1610-TW) |
Readout System |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
TNF-related weak inducer of apoptosis receptor (TWEAK R) belongs to the TNF receptor superfamily and is designated TNFRSF12. The gene for TWEAK R was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Mouse TWEAK R cDNA encodes a 129 amino acid (aa) residues type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain, and a 28 aa cytoplasmic domain (1-3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain contains one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death by both caspase-dependent apoptosis and cathepsin B-dependent necrosis, cell proliferation, and angiogenesis (2-6). In newborn mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung, and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).
Secondary Antibodies |
Isotype Controls |
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