![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (TPSAB1/1961) [NBP2-75751] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Tryptase Mouse Monoclonal Antibody (TPSAB1/1961).](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-TPSAB1-1961-Immunohistochemistry-Paraffin-NBP2-75751-img0001.jpg "Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (TPSAB1/1961) [NBP2-75751] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Tryptase Mouse Monoclonal Antibody (TPSAB1/1961).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | TPSAB1/1961 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75754) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human Tryptase alpha/TPS1 protein fragment (around aa 115-233) (Exact sequence is proprietary) (Uniprot: Q15661) |
| Localization | Secreted and Cytoplasmic |
| Marker | Mast Cell Marker |
| Specificity | This antibody reacts with mast cells distributed in skin, synovium, lung, and heart. This antibody does not bind with any other cell type. Human mast cell tryptase is considered to be an important marker of mast cell activation and is an important mediator of inflammation. Mastocytosis is a term collectively used for a group of disorders in which there is abnormal accumulation of mast cells in one or multiple organs. Anti-tryptase, combined with anti-CD2, anti-CD25, and anti-CD117, can be useful in identifying reactive mast cell hyperplasia, myelogenous neoplasms, mast cell leukemia, and mastocytosis. Due to sequence identity this antibody will likely cross react with tryptase beta 2. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TPSAB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Tryptase alpha/TPS1 Antibody (NBP2-75751)Find related products by research area.
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![Protein Array: Tryptase alpha/TPS1 Antibody (TPSAB1/1961) [NBP2-75751] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Tryptase alpha/TPS1 Antibody (TPSAB1/1961). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-TPSAB1-1961-Protein-Array-NBP2-75751-img0003.jpg "Protein Array: Tryptase alpha/TPS1 Antibody (TPSAB1/1961) [NBP2-75751] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Tryptase alpha/TPS1 Antibody (TPSAB1/1961). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
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