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Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]

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 (2 Reviews)
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Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Chymase/CMA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG ...read more
Recombinant Human Chymase/CMA1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (MAB40991) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
422818
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
MAB4099-SP
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Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chymase/CMA1
Gly20-Asn247
Accession # P23946
Specificity
Detects human Chymase/CMA1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) MCPT-1, rmMCPT-6, rmMCPT-7, or rmMCPT-11.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CMA1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
MAB4099 in the following applications:
Publications
Read Publications using
MAB4099 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]

  • Alpha-chymase
  • chymase 1 preproprotein transcript E
  • chymase 1 preproprotein transcript I
  • chymase 1, mast cell
  • Chymase
  • chymase, heart
  • chymase, mast cell
  • CMA1
  • CYH
  • CYM
  • EC 3.4.21
  • EC 3.4.21.39
  • Mast cell protease I
  • MCP3P
  • Mcpt5
  • MCT1
  • MGC119890
  • MGC119891

Background

Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2‑residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.

  1. Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. ed. p. 1531, Academic Press, San Diego.
  2. Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
  3. Miyazaki, M. and S. Takai (2006) J. Pharmacol. Sci. 100:391.
  4. Nakajima, M. and N. Naya (2002) Jpn. J. Pharmacol. 90:206.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chymase/CMA1/Mast Cell Chymase Antibody (MAB4099)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Human
(1)
Rat
(1)
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Reviews for Chymase/CMA1/Mast Cell Chymase Antibody (MAB4099) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB4099:
Filter by Applications
WB
(2)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Human 02/20/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22180785
SpeciesHuman
  5
reviewed by:
Verified Customer
 WB Human 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23312967
SpeciesHuman

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FAQs for Chymase/CMA1/Mast Cell Chymase Antibody (MAB4099) (0)

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Recent Reviews

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 Verified Customer
02/20/2015
Application: WB
Species: Human
read this review
 
 Verified Customer
02/20/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol CMA1
Uniprot