![Western Blot: Transglutaminase 5 Antibody [NBP2-94524] - Analysis of extracts of various cell lines, using Transglutaminase 5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.](http://images.novusbio.com/fullsize/Transglutaminase-5-Antibody-Western-Blot-NBP2-94524-img0004.jpg "Western Blot: Transglutaminase 5 Antibody [NBP2-94524] - Analysis of extracts of various cell lines, using Transglutaminase 5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 461-720 of human TGM5 (NP_963925.2). LQKLKARSFHGSQRGAELQPSRPTSLSQDSPRSLHTPSLRPSDVVQVSLKFKLLDPPNMGQDICFVLLALNMSSQFKDLKVNLSAQSLLHDGSPLSPFWQDTAFITLSPKEAKTYPCKISYSQYSQYLSTDKLIRISALGEEKSSPEKILVNKIITLSYPSITINVLGAAVVNQPLSIQVIFSNPLSEQVEDCVLTVEGSGLFKKQQKVFLGVLKPQHQASIILETVPFKSGQRQIQANMRSNKFKDIKGYRNVYVDFAL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TGM5 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Western Blot Transglutaminase 7/TGM7 Antibody [Unconjugated]](https://images.novusbio.com/images2/Transglutaminase_7_AF5426_Western_Blot_12074.jpg)
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| Publication using NBP2-94524 | Applications | Species |
|---|---|---|
| Lee WS, Al-Ramahi I, Jeong HH et al. Cross-species genetic screens identify transglutaminase 5 as a regulator of polyglutamine-expanded ataxin-1 The Journal of clinical investigation May 2 2022 [PMID: 35499073] (WB, IHC-P, Mouse, Human) | WB, IHC-P | Mouse, Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Transglutaminase 5 Antibody (NBP2-94524)Find related products by research area.
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| Gene Symbol | TGM5 |
![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded rat kidney using Transglutaminase 5 .](http://images.novusbio.com/fullsize/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0003.jpg "Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded rat kidney using Transglutaminase 5 .")
![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded human colon using Transglutaminase 5 .](http://images.novusbio.com/fullsize/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0001.jpg "Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded human colon using Transglutaminase 5 .")
![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded mouse heart using Transglutaminase 5 .](http://images.novusbio.com/fullsize/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0002.jpg "Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded mouse heart using Transglutaminase 5 .")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")