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Transglutaminase 3/TGM3 Antibody

Images

 
Western Blot: Transglutaminase 3/TGM3 Antibody [NBP1-86950] - Analysis in human cell line EFO-21.
Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950] - Staining of human esophagus shows high expression.
Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950] - Staining of human lymph node shows no positivity in germinal center cells and non-germinal center cells as expected.
Immunohistochemistry-Paraffin: Transglutaminase 3/TGM3 Antibody [NBP1-86950] - Staining of human skin shows weak cytoplasmic positivity in squamous epithelial cells.
Transglutaminase 3/TGM3 Antibody [NBP1-86950]-Staining of human skeletal muscle shows negative cytoplasmic positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Transglutaminase 3/TGM3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GSDQERQVFQKALGKLKPNTPFAATSSMGLETEEQEPSIIGKLKVAGMLAVGKEVNLVLLLKNLSRDTKTVTVNMTAWTIIYNGTLVHEVWKDSATMSLDPEEEAEHPIKISYAQYEKYLKSDNMI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGM3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Transglutaminase 3/TGM3 Protein (NBP1-86950PEP)
Publications
Read Publications using
NBP1-86950 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Transglutaminase 3/TGM3 Antibody

  • EC 2.3.2.13
  • MGC126250
  • protein-glutamine gamma-glutamyltransferase E
  • TG(E)
  • TG3
  • TGase E
  • TGase-3
  • TgaseE
  • TGEMGC126249
  • TGM3
  • transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase)
  • Transglutaminase 3
  • Transglutaminase E
  • transglutaminase-3

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent. Transglutaminase 3 consists of two polypeptide chains activated from a single precursor protein by proteolysis. Transglutaminase 3 is involved the later stages of cell envelope formation in the epidermis and hair follicle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Transglutaminase 3/TGM3 Antibody (NBP1-86950)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TGM3