![Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cytokeratin-10-Antibody-3C2F5-Western-Blot-NBP2-61736-img0006.jpg "Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clone | 3C2F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Cytokeratin 10 (AA: 345-454) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT10 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 58.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
| Publications using NBP2-61736 | Applications | Species |
|---|---|---|
| Wang Z, Xu H, Yang H et al. Single-stage transplantation combined with epidermal stem cells promotes the survival of tissue-engineered skin by inducing early angiogenesis Stem cell research & therapy 2023-03-23 [PMID: 36959609] (FLOW, IHC-P, Rat) Details: Dilution used in Flow 1:50, in IHC-P 1:200 |
FLOW, IHC-P | Rat |
| Xu h, Wang Z, Yang H et al. Epidermal stem cell-derived exosomes alleviate autophagy induced endothelial cell apoptosis through miR200b-3p/ERK pathway in diabetic wounds Research Square 2022-06-16 (FLOW, Mouse) | FLOW | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 10 Antibody (NBP2-61736)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT10 |
![Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of esophageal cancer tissues using KRT10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-10-Antibody-3C2F5-Immunohistochemistry-NBP2-61736-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of esophageal cancer tissues using KRT10 mouse mAb with DAB staining.")
![Flow Cytometry: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cytokeratin-10-Antibody-3C2F5-Flow-Cytometry-NBP2-61736-img0002.jpg "Flow Cytometry: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).")
![Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against human KRT10 (AA: 345-454) recombinant protein. (Expected MW is 38.7 kDa)](http://images.novusbio.com/fullsize/Cytokeratin-10-Antibody-3C2F5-Western-Blot-NBP2-61736-img0005.jpg "Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against human KRT10 (AA: 345-454) recombinant protein. (Expected MW is 38.7 kDa)")
![Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mouse mAb against A431 (1), C6 (2), COS7 (3), Jurkat (4), NIH/3T3 (5), and HEK293 (6) cell lysate.](http://images.novusbio.com/fullsize/Cytokeratin-10-Antibody-3C2F5-Western-Blot-NBP2-61736-img0007.jpg "Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mouse mAb against A431 (1), C6 (2), COS7 (3), Jurkat (4), NIH/3T3 (5), and HEK293 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of breast cancer tissues using KRT10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-10-Antibody-3C2F5-Immunohistochemistry-NBP2-61736-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of breast cancer tissues using KRT10 mouse mAb with DAB staining.")
![ELISA: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Cytokeratin-10-Antibody-3C2F5-ELISA-NBP2-61736-img0001.jpg "ELISA: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")