Novus Biologicals products are now on bio-techne.com

Transferrin Native Protein

Images

 
Dot Blot: Transferrin Native Protein [NBP1-96857] - Dot Blot of Rhodamine Conjugated Human Transferrin. Dotted directly with Rhodamine Conjugated Human Transferrin at following concentrations. Load: Lane 1 - 50ng Lane 2 ...read more
Dot Blot of Rhodamine Conjugated Human Transferrin. Dotted directly with Transferrin Native Proteinat following concentrations. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications DB
Conjugate
Rhodamine
Concentration
LYOPH

Order Details

View Available Conjugates

Transferrin Native Protein Summary

Description
Native Human Transferrin

Source: Normal serum (Accession #:Q06AH7)

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Preparation
Method
This product was prepared from normal serum by delipidation, salt fractionation, selective precipitation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Transferrin and anti-Human Serum.
Protein/Peptide Type
Native Protein
Gene
TF
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Dot Blot
Application Notes
This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Alternate Names for Transferrin Native Protein

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-41532
Species: Mu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP3-15868
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
MEP00B
Species: Mu
Applications: ELISA
DHAPG0
Species: Hu
Applications: ELISA
236-EG
Species: Hu
Applications: BA
DY1707
Species: Hu
Applications: ELISA
DTFP10
Species: Hu
Applications: ELISA
NBP1-05027
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP1-83250
Species: Hu
Applications: IHC, IHC-P, WB
M6000B
Species: Mu
Applications: ELISA
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-33320
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
202-IL
Species: Hu
Applications: BA
AF1148
Species: Hu
Applications: CyTOF-ready, Flow, Simple Western, WB
NBP1-96857
Species: Hu
Applications: DB

Publications for Transferrin Native Protein (NBP1-96857) (0)

There are no publications for Transferrin Native Protein (NBP1-96857).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Transferrin Native Protein (NBP1-96857) (0)

There are no reviews for Transferrin Native Protein (NBP1-96857). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Transferrin Native Protein (NBP1-96857) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Transferrin Products

Diseases for Transferrin Native Protein (NBP1-96857)

Discover more about diseases related to Transferrin Native Protein (NBP1-96857).
 

Pathways for Transferrin Native Protein (NBP1-96857)

View related products by pathway.

PTMs for Transferrin Native Protein (NBP1-96857)

Learn more about PTMs related to Transferrin Native Protein (NBP1-96857).
 

Research Areas for Transferrin Native Protein (NBP1-96857)

Find related products by research area.

Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Transferrin Native Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol TF
Uniprot