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TORC2 Antibody

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Western Blot: TORC2 Antibody [NBP1-86926] - Analysis in human cell line THP-1.
Western Blot: TORC2 Antibody [NBP1-86926] - Analysis in human cell line THP-1.
Immunocytochemistry/ Immunofluorescence: TORC2 Antibody [NBP1-86926] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: TORC2 Antibody [NBP1-86926] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Western Blot: TORC2 Antibody [NBP1-86926] - Analysis in control (vector only transfected HEK293T lysate) and CRTC2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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TORC2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SPSASLVLDPPGFSEGPGFLGGEGPMGGPQDPHTFNHQNLTHCSRHGSGPNIILTGDSSPGFSKEIAAALAGVPGFEVSAAGLELGLGL
Predicted Species
Mouse (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRTC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TORC2 Protein (NBP1-86926PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TORC2 Antibody

  • CREB regulated transcription coactivator 2
  • CREB-regulated transcription coactivator 2
  • CRTC2
  • TORC2
  • TORC-2
  • TORC2transducer of regulated cAMP response element-binding protein (CREB) 2
  • Transducer of CREB protein 2
  • Transducer of regulated cAMP response element-binding protein 2

Background

TORC2 (transducer of regulated CREB activity 2) is part of a family of CREB (cAMP response element-binding protein) coactivators that also includes TORC1 and TORC3. These proteins function to activate CREB-dependent transcription in response to cAMP and calcium signaling pathways. TORC2 has been found to be pivotal to the process of gluconeogenesis. TORC2 is also known as CREB regulated transcription activator 2, and CRTC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CRTC2