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TOMM34 Antibody

Images

 
Western Blot: TOMM34 Antibody [NBP2-38810] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver ...read more
Immunocytochemistry/ Immunofluorescence: TOMM34 Antibody [NBP2-38810] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunohistochemistry-Paraffin: TOMM34 Antibody [NBP2-38810] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry: TOMM34 Antibody [NBP2-38810] - Staining of human testis shows strong cytoplasmic positivity in basal cells of seminiferous ducts.
Orthogonal Strategies: Immunohistochemistry-Paraffin: TOMM34 Antibody [NBP2-38810] - Staining in human testis and endometrium tissues using anti-TOMM34 antibody. Corresponding TOMM34 RNA-seq data are presented ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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TOMM34 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NVTSAVEGINRMTRALMDSLGPEWRLKLPSIPLVPVSAQKRWNSLPSENHKEMAKSKSKETTATKNRVPSAG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TOMM34
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TOMM34 Protein (NBP2-38810PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TOMM34 Antibody

  • hTom34
  • HTOM34P
  • mitochondrial import receptor subunit TOM34
  • outer mitochondrial membrane translocase (34kD)
  • TOM34
  • Translocase of outer membrane 34 kDa subunit
  • translocase of outer mitochondrial membrane 34
  • URCC3

Background

TOMM34 is encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TOMM34
Uniprot