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TRIM29 Antibody

Images

 
Western Blot: TRIM29 Antibody [NBP1-88875] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: TRIM29 Antibody [NBP1-88875] - Staining of human cell line SiHa shows localization to intermediate filaments.
Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-88875] - Staining of human skin shows cytoplasmic positivity in epidermal cells.
Western Blot: TRIM29 Antibody [NBP1-88875] - Analysis in human tonsil tissue.
Western Blot: TRIM29 Antibody [NBP1-88875] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-88875] - Staining of human esophagus shows high expression.
Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-88875] - Staining of human stomach shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-88875] - Staining in human esophagus and stomach tissues using anti-TRIM29 antibody. Corresponding TRIM29 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-88875] - Staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-88875] - Staining of human prostate shows cytoplasmic positivity in basal layer of glandular cells.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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TRIM29 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GDDKNSNYFSMDSMEGKRSPYAGLQLGAAKKPPVTFAEKGELRKSIFSESRKPTVSIMEPGETRRNSYPRADTGLFSRSKSGSEEVLCDSCIGNKQKAVKSCLVCQASFCELHL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM29
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TRIM29 Recombinant Protein Antigen (NBP1-88875PEP)

Reactivity Notes

Mouse (87%), Rat (87%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRIM29 Antibody

  • Ataxia telangiectasia group D-associated protein
  • ATDC ataxia-telangiectasia group D-associated protein
  • ATDC
  • FLJ36085
  • tripartite motif containing 29
  • tripartite motif protein TRIM29
  • tripartite motif-containing 29
  • tripartite motif-containing protein 29

Background

TRIM29 is encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRIM29