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TNF-alpha Antibody [Unconjugated]

Images

 
Recombinant Rat TNF‑ alpha (Catalog # 510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by ...read more
TNF-a was detected in immersion fixed rat splenocytes using Goat Anti-Rat TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
Astrocytes activity and GFAP/TNF-alpha double staining in the DG area.Astrocyte activity was measure with GFAP immunofluorescence. Representative photomicrographs show control and LPS-injected rats at different time ...read more
Astrocytes activity and GFAP/TNF-alpha double staining in the DG area.Astrocyte activity was measure with GFAP immunofluorescence. Representative photomicrographs show control and LPS-injected rats at different time ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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TNF-alpha Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant rat TNF-alpha
Leu80-Leu235
Accession # P16599
Specificity
Detects rat TNF‑ alpha in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant porcine TNF-alpha is observed and less than 1% cross-reactivity with recombinant human (rh) TNF-beta , rhTNF sRI, and rhTNF sRII is observed. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine TNF-alpha , recombinant feline TNF-alpha , and recombinant rhesus macaque TNF-alpha is observed. Neutralizes the biological activity of rat TNF-alpha . This antibody will also neutralize the biological activity of murine TNF-alpha , but weak neutralization of rhTNF-alpha and no neutralization of rhTNF-beta was observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNF
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.15-0.75 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF-510-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody [Unconjugated]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor Necrosis Factor Alpha (TNF-alpha ) also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69% - 76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha . The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

  1. Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Estler, H.C. et al. (1992) Biol. Chem. Hoppe-Seyler 373:271.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  9. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335.
  10. Romanatto, T. et al. (2007) Peptides 28:1050.
  11. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF-alpha Antibody (AF-510-NA)(50)

We have publications tested in 2 confirmed species: Rat, Transgenic Rat.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(4)
IHC
(9)
IHC-Fr
(4)
IHC-P
(3)
Neutralization
(11)
Western Blot
(7)
All Applications
Filter By Species
Rat
(33)
Transgenic Rat
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using AF-510-NA Applications Species
Hao Tang, Xuemei Zhang, Xiaojun Hao, Haitong Dou, Chendan Zou, Yinglian Zhou et al. Hepatocyte growth factor-modified hair follicle stem cells ameliorate cerebral ischemia/reperfusion injury in rats Stem Cell Research & Therapy 2/13/2023 [PMID: 36782269]
A Nagayach, R Bhaskar, I Patro Microglia activation and inflammation in hippocampus attenuates memory and mood functions during experimentally induced diabetes in rat Journal of chemical neuroanatomy, 2022-09-08;0(0):102160. 2022-09-08 [PMID: 36089179] (IHC, Rat) IHC Rat
QY Li, SX Chen, JY Liu, PW Yao, YW Duan, YY Li, Y Zang Neuroinflammation in the anterior cingulate cortex: the potential supraspinal mechanism underlying the mirror-image pain following motor fiber injury Journal of Neuroinflammation, 2022-06-20;19(1):162. 2022-06-20 [PMID: 35725625] (Neutralization, Rat) Neutralization Rat
T Xu, X Li, Y Guo, E Uhlin, L Holmberg, S Mitra, D Winn, A Falk, E Sundström Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia EBioMedicine, 2022-02-16;77(0):103882. 2022-02-16 [PMID: 35182996] (IHC, Rat) IHC Rat
Fei Cheng, Jing Liu, Zhuolin Guo, Shicheng Li, Jingfu Chen, Chang Tu et al. Angiotensin-(1-7) ameliorates high glucose-induced vascular endothelial injury through suppressing chloride channel 3 Bioengineered 2/15/2022 [PMID: 35098884]
ZH Wang, Y Xia, Z Wu, SS Kang, JC Zhang, P Liu, X Liu, W Song, V Huin, CM Dhaenens, SP Yu, XC Wang, K Ye Neuronal ApoE4 stimulates C/EBPbeta activation, promoting Alzheimer&#039;s disease pathology in a mouse model Progress in neurobiology, 2021-12-24;209(0):102212. 2021-12-24 [PMID: 34958873] (IHC, Rat) IHC Rat
T Balzano, P Leone, G Ivaylova, MC Castro, L Reyes, C Ramón, M Malaguarne, M Llansola, V Felipo Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage Biomedicines, 2021-08-12;9(8):. 8/12/2021 [PMID: 34440206]
Michele Malaguarnera, Tiziano Balzano, Mari Carmen Castro, Marta Llansola, Vicente Felipo The Dual Role of the GABAA Receptor in Peripheral Inflammation and Neuroinflammation: A Study in Hyperammonemic Rats International Journal of Molecular Sciences 6/24/2021 [PMID: 34202516]
Momoko Koizumi, Sayaka Asano, Akihiko Furukawa, Yoshinori Hayashi, Suzuro Hitomi, Ikuko Shibuta et al. P2X3 receptor upregulation in trigeminal ganglion neurons through TNF alpha production in macrophages contributes to trigeminal neuropathic pain in rats The Journal of Headache and Pain 12/1/2021 [PMID: 33902429]
María del Mar Fernández-Arjona, Ana León-Rodríguez, María Dolores López-Ávalos, Jesús M. Grondona Microglia activated by microbial neuraminidase contributes to ependymal cell death Fluids and Barriers of the CNS 3/23/2021 [PMID: 33757539]
Show All 50 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TNF-alpha Antibody (AF-510-NA). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies

 

Isotype Controls

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

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Bioinformatics

Gene Symbol TNF
Uniprot