I need to know about TNF-alpha antibody kits for mice.

We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.

I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.

I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.

I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?

The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.

Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?

I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Novus Biologicals products are now on bio-techne.com

TNF-alpha Antibody (28401) [Unconjugated]

Name: TNF-alpha Antibody (28401) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB610
Rating: 5 (1 reviews)

(70 Publications)

(1 Review)

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Western blot shows 25 ng of Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Rat TNF-a (Catalog # 510-RT), and Recombinant Human APRIL/TNFSF13 (Catalog # ...read more

TNF-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610) at 10 µg/mL for 3 hours at ...read more

Recombinant Human TNF‑ alpha (Catalog #210‑TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha ...read more

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB, B/N, ELISA(Cap), ICC/IF
Clone	28401
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Order Details

View Available Formulations

Catalog# & Formulation	Size	Price
Carrier Free MAB610-SP

Carrier Free MAB610-500

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TNF-alpha Antibody (28401) [Unconjugated] Summary

Immunogen	E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375
Specificity	Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF‑ beta , recombinant mouse TNF‑ alpha , recombinant rat TNF‑ alpha , and recombinant porcine TNF‑ alpha is observed.
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	TNF
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
Immunocytochemistry 8-25 ug/mL
Neutralization 0.01-0.04 ug/mL
Western Blot 1 ug/mL

Application Notes

ELISA Detection: Human TNF-alpha Biotinylated Antibody (Catalog number BAF210)
Standard: Recombinant Human TNF-alpha (Catalog number 210-TA)

Reviewed Applications

Read 1 Review rated 5

using
MAB610 in the following applications:

Western Blot

Publications

Read Publications using
MAB610 in the following applications:

Affinity Chromatography
1 publication
Antibody Array Development
1 publication
Array Development
1 publication
B/N
1 publication
Bioassay
2 publications
ELISA Capture
1 publication
ELISA Development
11 publications
ELISA Development (Capture)
1 publication
ELISA Microarray Development
1 publication
Electrochemiluminescent Assay
1 publication
ICC
2 publications
IHC
4 publications
IHC-Fr
3 publications
IHC-P
1 publication
Immunoprecipitation
1 publication
Luminex Development
3 publications
Neutralization
11 publications
Western Blot
3 publications

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody (28401) [Unconjugated]

APC1 protein
Cachectin
Cachetin
DIF
TNF
TNF, monocyte-derived
TNFA
TNF-A
TNFalpha
TNF-alpha
TNF-alphacachectin
TNFATNF, macrophage-derived
TNFG1F
TNFSF1A
TNFSF2
TNFSF2TNF superfamily, member 2
tumor necrosis factor (TNF superfamily, member 2)
tumor necrosis factor alpha
Tumor necrosis factor ligand superfamily member 2
tumor necrosis factor
tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha , TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF- is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF-alpha Antibody (MAB610)(70)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 18 applications: Affinity Chromatography, Antibody Array Development, Array Development, B/N, Bioassay, ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Microarray Development, Electrochemiluminescent Assay, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Luminex Development, Neutralization, Western Blot.

Showing Publications 1 - 10 of 70. Show All 70 Publications.

Publications using MAB610	Applications	Species
Abu El-Asrar, AM;Nawaz, MI;Ahmad, A;Dillemans, L;Siddiquei, M;Allegaert, E;Gikandi, PW;De Hertogh, G;Opdenakker, G;Struyf, S; CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy International journal of molecular sciences 2023-10-25 [PMID: 37958563] (Western Blot, Human)	Western Blot	Human
Kim, JK;Chang, I;Jung, Y;Kaplan, Z;Hill, EE;Taichman, RS;Krebsbach, PH; Mycoplasma hyorhinis infection promotes TNF-? signaling and SMAC mimetic-mediated apoptosis in human prostate cancer Heliyon 2023-10-01 [PMID: 37867861] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Lip Ket Chin, Jun-Yeong Yang, Benjamin Chousterman, Sunghoon Jung, Do-Geun Kim, Dong-Ho Kim et al. Dual-Enhanced Plasmonic Biosensing for Point-of-Care Sepsis Detection ACS Nano 2/28/2023 [PMID: 36745820]
P Kantaputra, T Daroontum, M Chuamanoch, S Chaowattan, W Intachai, B Olsen, T Sastraruji, S Tongsima, C Ngamphiw, J Kampuansai, TC Cox, S Kiratikano Loss of Function TGFBR2 Variant as a Contributing Factor in Generalized Pustular Psoriasis and Adult-Onset Immunodeficiency Genes, 2022-12-29;14(1):. 2022-12-29 [PMID: 36672844] (IHC, Human)	IHC	Human
RF Iyer, DM Edwards, P Kolb, HP Raué, CA Nelson, ML Epperson, MK Slifka, JC Nolz, H Hengel, DH Fremont, K Früh The secreted protein Cowpox Virus 14 contributes to viral virulence and immune evasion by engaging Fc-gamma-receptors PloS Pathogens, 2022-09-19;18(9):e1010783. 2022-09-19 [PMID: 36121874] (Bioassay, Human)	Bioassay	Human
Li Z, Fu WJ, Chen XQ et al. Autophagy-based unconventional secretion of HMGB1 in glioblastoma promotes chemosensitivity to temozolomide through macrophage M1-like polarization Journal of experimental & clinical cancer research : CR 2/22/2022 [PMID: 35193644]
Tanya Singh, Adam Neal, Gabriella Dibernardo, Neela Raheseparian, Neda A. Moatamed, Sanaz Memarzadeh Efficacy of birinapant in combination with carboplatin in targeting platinum‑resistant epithelial ovarian cancers International Journal of Oncology 2/21/2022 [PMID: 35191515]
S Cataldi, M Aprile, D Melillo, I Mucel, S Giorgetti-, M Cormont, P Italiani, M Blüher, JF Tanti, A Ciccodicol, V Costa TNFalpha Mediates Inflammation-Induced Effects on PPARG Splicing in Adipose Tissue and Mesenchymal Precursor Cells Cells, 2021-12-24;11(1):. 2021-12-24 [PMID: 35011604] (Neutralization, Human)	Neutralization	Human
TPB De Luccia, E Ono, R Menon, AU Borbely, R Mattar, L Richardson, ALM da Silva, RM Botelho, MLTLF da Rocha, S Daher The effect of Gestational Diabetes Mellitus on the fetal compartment Journal of reproductive immunology, 2021-03-24;145(0):103314. 2021-03-24 [PMID: 33836321] (IHC, Human)	IHC	Human
Darcy R Denner, Maria LD Udan-Johns, Michael R Nichols Inhibition of matrix metalloproteinase-9 secretion by dimethyl sulfoxide and cyclic adenosine monophosphate in human monocytes World Journal of Biological Chemistry 1/27/2021 [PMID: 33552397]
Arpita Saha, Hamdi Ben Halima, Abhishek Saini, Juan Gallardo-Gonzalez, Nadia Zine, Clara Viñas et al. Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva Molecules 8/31/2020 [PMID: 32878151]
T Pan, F Zhang, F Li, X Gao, Z Li, X Li, X Ren Shikonin blocks human lung adenocarcinoma cell migration and invasion in the inflammatory microenvironment via the IL?6/STAT3 signaling pathway Oncol. Rep., 2020-07-09;44(3):1049-1063. 2020-07-09 [PMID: 32705271] (B/N, Neutralization, Human)	B/N, Neutralization	Human
HN Khan, X Brands, S Aufiero, AJ Hoogendijk, AM Klarenbeek, TSR van Engele, BW Haak, LA van Vught, J Horn, MJ Schultz, AH Zwinderman, T van der Po, BP Scicluna The circular RNA landscape in specific peripheral blood mononuclear cells of critically ill patients with sepsis Crit Care, 2020-07-13;24(1):423. 2020-07-13 [PMID: 32660590] (Human)		Human
Jing Liu, Guang Song, Tao Meng, Ge Zhao UL16-Binding Protein 1 Induced HTR-8/SVneo Autophagy via NF-kappa B Suppression Mediated by TNF-alpha Secreted through uNK Cells BioMed Research International 6/13/2020 [PMID: 32626772]
J Seo, D Seong, YW Nam, CH Hwang, SR Lee, CS Lee, Y Jin, HW Lee, DB Oh, P Vandenabee, J Song Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex Cell Death Differ., 2020-05-26;0(0):. 2020-05-26 [PMID: 32457484] (Neutralization, Mouse)	Neutralization	Mouse
N Lalaoui, D Merino, G Giner, F Vaillant, D Chau, L Liu, T Kratina, B Pal, JR Whittle, N Etemadi, J Berthelet, J Gräsel, C Hall, ME Ritchie, M Ernst, GK Smyth, DL Vaux, JE Visvader, GJ Lindeman, J Silke Targeting triple-negative breast cancers with the Smac-mimetic birinapant Cell Death Differ., 2020-04-27;0(0):. 2020-04-27 [PMID: 32341449] (Neutralization, Human)	Neutralization	Human
Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi et al. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours Transplantation 9/1/2020 [PMID: 32168040]
Davorka Herman Herman Mahečić, Maja Cigrovski Cigrovski Berković, Vanja Zjačić-Rotkvić, Tamara Čačev, Sanja Kapitanović, Monika Ulamec Inflammation-related cytokines and their roles in gastroenteropancreatic neuroendocrine neoplasms Bosnian Journal of Basic Medical Sciences 11/2/2020 [PMID: 32156252]
A Stumpf, T Brandstett, J Hübner, J Rühe Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum PLoS ONE, 2019-12-02;14(12):e0225525. 2019-12-02 [PMID: 31790441] (ELISA Capture, Human)	ELISA Capture	Human
E Hoste, C Maueröder, L van Hove, L Catrysse, HK Vikkula, M Sze, B Maes, D Karjosukar, L Martens, A Gonçalves, E Parthoens, R Roelandt, W Declercq, I Fuentes, F Palisson, S Gonzalez, JC Salas-Alan, L Boon, P Huebener, KW Mulder, K Ravichandr, Y Saeys, RF Schwabe, G van Loo Epithelial HMGB1 Delays Skin Wound Healing and Drives Tumor Initiation by Priming Neutrophils for NET Formation Cell Rep, 2019-11-26;29(9):2689-2701.e4. 2019-11-26 [PMID: 31775038] (IHC, Mouse)	IHC	Mouse
Paramita Baruah, Jessica Bullenkamp, Philip O. G. Wilson, Michael Lee, Juan Carlos Kaski, Ingrid E. Dumitriu TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2 Frontiers in Immunology 7/16/2019 [PMID: 31379843]
Mancy Tong, Julie A. Potter, Gil Mor, Vikki M. Abrahams Lipopolysaccharide-Stimulated Human Fetal Membranes Induce Neutrophil Activation and Release of Vital Neutrophil Extracellular Traps The Journal of Immunology 7/15/2019 [PMID: 31167775]
W Sun, N Meednu, A Rosenberg, J Rangel-Mor, V Wang, J Glanzman, T Owen, X Zhou, H Zhang, BF Boyce, JH Anolik, L Xing B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation Nat Commun, 2018-12-03;9(1):5127. 2018-12-03 [PMID: 30510188] (Neutralization, Mouse)	Neutralization	Mouse
Y Wang, J Liu, X Chen, H Sun, S Peng, Y Kuang, J Pi, T Zhuang, L Zhang, Z Yu, B Tomlinson, P Chan, Y Chen, Y Zhang, Y Li Dysfunctional endothelial-derived microparticles promote inflammatory macrophage formation via NF-?B and IL-1? signal pathways J. Cell. Mol. Med., 2018-10-18;0(0):. 2018-10-18 [PMID: 30334371] (Western Blot, Human)	Western Blot	Human
Michela Frascoli, Lacy Coniglio, Russell Witt, Cerine Jeanty, Shannon Fleck-Derderian, Dana E. Myers et al. Alloreactive fetal T cells promote uterine contractility in preterm labor via IFN-gamma and TNF-alpha Science Translational Medicine 4/25/2018 [PMID: 29695455]
Rikard Pehrson, Tove Hegelund-Myrbäck, Danen Cunoosamy, Sara Asimus, Paul Jansson, Naimish Patel et al. AZD7624, an Inhaled p38 Inhibitor, Demonstrates Local Lung Inhibition of LPS-Induced TNF alpha with Minimal Systemic Exposure Journal of Pharmacology and Experimental Therapeutics 6/1/2018 [PMID: 29549158]
S Urra, MC Fischer, JR Martínez, L Véliz, P Orellana, A Solar, K Bohmwald, A Kalergis, C Riedel, AH Corvalán, JC Roa, R Fuentealba, CJ Cáceres, M López-Last, A León, N Droppelman, HE González Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis? Oncotarget, 2017-12-20;9(2):2445-2467. 2017-12-20 [PMID: 29416784] (Western Blot, Human)	Western Blot	Human
K Ouhara, S Munenaga, M Kajiya, K Takeda, S Matsuda, Y Sato, Y Hamamoto, T Iwata, S Yamasaki, K Akutagawa, N Mizuno, T Fujita, E Sugiyama, H Kurihara The induced RNA-binding protein, HuR targets 3'-UTR region of IL-6 mRNA and enhances its stabilization in periodontitis Clin. Exp. Immunol., 2018-03-08;0(0):. 2018-03-08 [PMID: 29393507] (Neutralization, Human)	Neutralization	Human
T Regan, AC Gill, SM Clohisey, MW Barnett, CM Pariante, NA Harrison, DA Hume, ET Bullmore, TC Freeman Effects of anti-inflammatory drugs on the expression of tryptophan-metabolism genes by human macrophages J. Leukoc. Biol., 2018-01-26;103(4):681-692. 2018-01-26 [PMID: 29377288] (Bioassay, Human)	Bioassay	Human
Vladimir N. Ivanov, Jinhua Wu, Tom K. Hei Regulation of human glioblastoma cell death by combined treatment of cannabidiol, gamma -radiation and small molecule inhibitors of cell signaling pathways Oncotarget 9/26/2017 [PMID: 29088769]
CM Henry, SJ Martin Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation Mol. Cell, 2017-02-16;65(4):715-729.e5. 2017-02-16 [PMID: 28212752] (ICC, Human)	ICC	Human
MC Tanzer, N Khan, JA Rickard, N Etemadi, N Lalaoui, SK Spall, JM Hildebrand, D Segal, M Miasari, D Chau, WL Wong, M McKinlay, SK Chunduru, CA Benetatos, SM Condon, JE Vince, MJ Herold, J Silke Combination of IAP antagonist and IFN? activates novel caspase-10- and RIPK1-dependent cell death pathways Cell Death Differ, 2017-01-20;0(0):. 2017-01-20 [PMID: 28106882] (Neutralization, Human)	Neutralization	Human
Jaime E. Castellanos, José I. Neissa, Sigrid J. Camacho La infección con el virus del dengue induce apoptosis en células del neuroblastoma humano SH-SY5Y Biomédica 4/15/2016 [PMID: 27622805]
H Jiang, X Zhang, H Lin Lysine fatty acylation promotes lysosomal targeting of TNF-? Sci Rep, 2016-04-15;6(0):24371. 2016-04-15 [PMID: 27079798] (IHC-Fr, Human)	IHC-Fr	Human
Matt P, Lindqvist U, Kleinau S Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcgammaR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment. PLoS ONE, 2015-09-25;10(9):e0137474. 2015-09-25 [PMID: 26406605] (ELISA Development (Capture), Human)	ELISA Development (Capture)	Human
Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips BMC Biotechnology 12/1/2015 [PMID: 26268325]
Zuo J, Shan Z, Zhou L, Yu J, Liu X, Gao Y Increased CD160 expression on circulating natural killer cells in atherogenesis. J Transl Med, 2015-06-13;13(0):188. 2015-06-13 [PMID: 26071079] (Neutralization, Human)	Neutralization	Human
Tudor C, Bruns H, Daniel C, Distel L, Hartmann A, Gerbitz A, Buettner M Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma. PLoS ONE, 2014-12-03;9(12):e114345. 2014-12-03 [PMID: 25470820] (Neutralization, Human)	Neutralization	Human
Elizabeth England, Philip Newton, Frances Neal, Lisa Kitching, Caroline Colley, Christine J. Rossant Application of the Mirrorball High-Sensitivity Cytometer to Multiplexed Assays for Antibody Drug Discovery J Biomol Screen 4/1/2015 [PMID: 25381256]
Eva Melin, Azita Sohrabian, Johan Rönnelid, Kristian Borg Normal serum levels of immune complexes in postpolio patients Results in Immunology 6/18/2014 [PMID: 25009767]
Aurelie Bornot, Carolyn Blackett, Ola Engkvist, Clare Murray, Claus Bendtsen The Role of Historical Bioactivity Data in the Deconvolution of Phenotypic Screens J Biomol Screen 6/1/2014 [PMID: 24441646]
Todd J, Simpson P, Estis J, Torres V, Wub A Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Cytokine, 2013-10-12;64(3):660-5. 2013-10-12 [PMID: 24128872] (ELISA Development, Human)	ELISA Development	Human
Chen X, Chang J, Deng Q et al. Progranulin Does Not Bind Tumor Necrosis Factor (TNF) Receptors and Is Not a Direct Regulator of TNF-Dependent Signaling or Bioactivity in Immune or Neuronal Cells. J Neurosci. 5/22/2013 [PMID: 23699531]
Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells. Clin Cancer Res, 2013-02-12;19(7):1784-94. 2013-02-12 [PMID: 23403634] (Neutralization, Human)	Neutralization	Human
Kaori Abe, Yoshiko Hashimoto, Shouki Yatsushiro, Shohei Yamamura, Mika Bando, Yuka Hiroshima et al. Simultaneous Immunoassay Analysis of Plasma IL-6 and TNF-alpha on a Microchip PLoS ONE 1/9/2013 [PMID: 23326472]
Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Cancer Res., 2011-11-07;72(1):66-75. 2011-11-07 [PMID: 22065722] (IHC-P, Human)	IHC-P	Human
Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia, 2011-07-09;54(10):2713-23. 2011-07-09 [PMID: 21744073] (ELISA Development, Human)	ELISA Development	Human
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF Protection from RNA and DNA viruses by IL-32. J. Immunol., 2011-02-23;186(7):4110-8. 2011-02-23 [PMID: 21346229] (Electrochemiluminescent Assay, Human)	Electrochemiluminescent Assay	Human
David C. Appleyard, Stephen C. Chapin, Patrick S. Doyle Multiplexed Protein Quantification with Barcoded Hydrogel Microparticles Analytical Chemistry 1/1/2011 [PMID: 21142122]
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-10-07;183(9):5799-806. 2009-10-07 [PMID: 19812208] (ELISA Development, Human)	ELISA Development	Human
Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)	Antibody Array Development	Human
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)	ELISA Microarray Development	Human
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (Luminex Development, Human)	Luminex Development	Human
Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J. Immunol., 2008-02-15;180(4):2553-61. 2008-02-15 [PMID: 18250466] (ELISA Development, Human)	ELISA Development	Human
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007-11-01;53(11):2010-2. 2007-11-01 [PMID: 18030696] (ELISA Development, Human)	ELISA Development	Human
John T, Muller RD, Oberholzer A, Zreiqat H, Kohl B, Ertel W, Hostmann A, Tschoeke SK, Schulze-Tanzil G Interleukin-10 modulates pro-apoptotic effects of TNF-alpha in human articular chondrocytes in vitro. Cytokine, 2007-11-19;40(3):226-34. 2007-11-19 [PMID: 18023359] (ICC, Human)	ICC	Human
Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose. J. Clin. Oncol., 2007-10-10;25(29):4542-9. 2007-10-10 [PMID: 17925549] (IHC-Fr, Human)	IHC-Fr	Human
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Ultrasensitive flow-based immunoassays using single-molecule counting. Clin. Chem., 2007-09-21;53(11):1990-5. 2007-09-21 [PMID: 17890441] (ELISA Development, Human)	ELISA Development	Human
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (Luminex Development, Human)	Luminex Development	Human
Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis. J. Pathol., 2007-05-01;212(1):47-55. 2007-05-01 [PMID: 17370327] (Neutralization, Human)	Neutralization	Human
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (Luminex Development, Human)	Luminex Development	Human
Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB Selective regulation of IL-10 signaling and function by zymosan. J. Immunol., 2006-04-15;176(8):4785-92. 2006-04-15 [PMID: 16585572] (ELISA Development, Human)	ELISA Development	Human
Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA Increased ENA-78 in the follicular fluid of patients with endometriosis. Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42. 2006-01-01 [PMID: 16553183] (ELISA Development, Human)	ELISA Development	Human
Hangai M, He S, Hoffmann S, Lim JI, Ryan SJ, Hinton DR Sequential induction of angiogenic growth factors by TNF-alpha in choroidal endothelial cells. J. Neuroimmunol., 2005-11-08;171(1):45-56. 2005-11-08 [PMID: 16288810] (IHC, Human)	IHC	Human
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5. 2004-12-01 [PMID: 15585763] (ELISA Development, Human)	ELISA Development	Human
Phillips TM Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Electrophoresis, 2004-06-01;25(10):1652-9. 2004-06-01 [PMID: 15188254] (Affinity Chromatography, Human)	Affinity Chromatography	Human
Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis. J. Immunol., 2004-05-15;172(10):6476-81. 2004-05-15 [PMID: 15128840] (ELISA Development, Human)	ELISA Development	Human
Buch MH, Conaghan PG, Quinn MA, Bingham SJ, Veale D, Emery P True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha? Ann. Rheum. Dis., 2004-03-19;63(10):1344-6. 2004-03-19 [PMID: 15033655] (IHC-Fr, Human)	IHC-Fr	Human
Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004-01-01;284(1):99-106. 2004-01-01 [PMID: 14736420] (Array Development, Human)	Array Development	Human
Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB Reprogramming of IL-10 activity and signaling by IFN-gamma. J. Immunol., 2003-11-15;171(10):5034-41. 2003-11-15 [PMID: 14607900] (ELISA Development, Human)	ELISA Development	Human
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Review for TNF-alpha Antibody (MAB610) (1) 51

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04/20/2017

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Application	Western Blot
Sample Tested	Human breast cancer cell lysate (MCF-7)
Species	Human
Lot	AVR1716011

Comments

MCF-7 cell extract probed with 1 ug/mL of Mouse anti-Human TNF-alpha Antibody (#MAB610) showing a specific band at 18 kDa. Membrane blocked with 5%BSA in PBS. Buffer: 5% BSA in PBS. Secondary Ab: anti-Mouse IgG 1:5,000.

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FAQs for TNF-alpha Antibody (MAB610). (Showing 1 - 4 of 4 FAQs).

I need to know about TNF-alpha antibody kits for mice.
- We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
- I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
- The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
- I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Blogs on TNF-alpha. Showing 1-10 of 13 blog posts - Show all blog posts.

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Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach... Read full blog post.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev... Read full blog post.

Read full blog post.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit... Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven of the Stanford University, CA, explained the relationship between four diseases:... Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN... Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t... Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

TNF-alpha [Unconjugated]
210-TA-005

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	Verified Customer 04/20/2017
Application:	WB
Species:	Human

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Gene Symbol	TNF
Uniprot	P01375()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

TNF-alpha Antibody (28401) [Unconjugated]

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Publications for TNF-alpha Antibody (MAB610)(70)

Review for TNF-alpha Antibody (MAB610) (1) 51

Summary

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Product General Protocols

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FAQs for TNF-alpha Antibody (MAB610). (Showing 1 - 4 of 4 FAQs).

Secondary Antibodies

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Additional TNF-alpha Products

Blogs on TNF-alpha. Showing 1-10 of 13 blog posts - Show all blog posts.

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