![Western Blot: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLE2.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Western-Blot-NBP2-74531-img0001.jpg "Western Blot: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLE2.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI5A6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human TLE2(NP_003251) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TLE2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 79.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
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![Neutralization Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Block_Neutralize_8975.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for TLE2 Antibody (NBP2-74531)Find related products by research area.
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| Gene Symbol | TLE2 |
![Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TLE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74531-img0010.jpg "Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TLE2 mouse monoclonal antibody.")
![Flow Cytometry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TLE2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74531-img0004.jpg "Flow Cytometry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TLE2 antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TLE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74531-img0005.jpg "Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TLE2 mouse monoclonal antibody.")
![Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TLE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74531-img0006.jpg "Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TLE2 mouse monoclonal antibody.")
![Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TLE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74531-img0007.jpg "Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TLE2 mouse monoclonal antibody.")
![Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TLE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74531-img0008.jpg "Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TLE2 mouse monoclonal antibody.")
![Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-TLE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74531-img0009.jpg "Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-TLE2 mouse monoclonal antibody.")
![Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-TLE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74531-img0011.jpg "Immunohistochemistry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-TLE2 mouse monoclonal antibody.")
![Flow Cytometry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Analysis of Hela cells, using anti-TLE2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74531-img0002.jpg "Flow Cytometry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Analysis of Hela cells, using anti-TLE2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Analysis of Jurkat cells, using anti-TLE2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TLE2-Antibody-OTI5A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74531-img0003.jpg "Flow Cytometry: TLE2 Antibody (OTI5A6) - Azide and BSA Free [NBP2-74531] - Analysis of Jurkat cells, using anti-TLE2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")