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HLF Antibody (M2)

Images

 
Western Blot: HLF Antibody (M2) [H00003131-M04] - Western blot analysis of HLF over-expressed 293 cell line, cotransfected with HLF Validated Chimera RNAi or non-transfected control. Blot probed with H00003131-M04. ...read more
Immunocytochemistry/ Immunofluorescence: HLF Antibody (M2) [H00003131-M04] - Immunofluorescence of to HLF ( Cat# H00003131-M04 ) on HeLa cell .
Western Blot: HLF Antibody (M2) [H00003131-M04] - analysis of HLF expression in transfected 293T cell line by HLF monoclonal antibody (M04), clone M2. Lane 1: HLF transfected lysate (33.2 KDa). Lane 2: Non-transfected ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
M2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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HLF Antibody (M2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
HLF (AAH36093, 1 a.a. ~ 295 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKMSRPLPLNPTFIPPPYGVLRSLLENPLKLPLHHEDAFSKDKDKEKKLDDESNSPTVPQSAFLGPTLWDKTLPYDGDTFQLEYMDLEEFLSENGIPPSPSQHDHSPHPPGLQPASSAAPSVMDLSSRASAPLHPGIPSPNCMQSPIRPGQLLPANRNTPSPIDPDTIQVPVGYEPDPADLALSSIPGQEMFDPRKRKFSEEELKPQPMIKKARKVFIPDDLKDDKYWARRRKNNMAAKRSRDARRLKENQIAIRASFLEKENSALRQEVADLRKELGKCKNILAKYEARHGPL
Specificity
HLF - hepatic leukemia factor
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLF
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
Publications
Read Publication using H00003131-M04.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HLF Antibody (M2)

  • hepatic leukemia factor
  • MGC33822

Background

This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLF Antibody (H00003131-M04)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HLF
Entrez
OMIM
Uniprot