![Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Western-Blot-NBP2-61621-img0007.jpg "Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clone | CL3573 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: GSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHR |
| Predicted Species | Mouse (95%), Rat (95%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TLE3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TLE3 Antibody (NBP2-61621)Find related products by research area.
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| Gene Symbol | TLE3 |
![Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEK 293](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Western-Blot-NBP2-61621-img0009.jpg "Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEK 293")
![Immunocytochemistry/Immunofluorescence: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of HeLa cells using the Anti-TLE3 monoclonal antibody, showing specific staining in the nucleoplasm and nucleoi in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Immunocytochemistry-Immunofluorescence-NBP2-61621-img0010.jpg "Immunocytochemistry/Immunofluorescence: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of HeLa cells using the Anti-TLE3 monoclonal antibody, showing specific staining in the nucleoplasm and nucleoi in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Immunohistochemistry-Paraffin-NBP2-61621-img0006.jpg "Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).")
![Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLa nuclear extract](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Western-Blot-NBP2-61621-img0008.jpg "Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLa nuclear extract")
![Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Immunohistochemistry-NBP2-61621-img0001.jpg "Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.")
![Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Immunohistochemistry-NBP2-61621-img0002.jpg "Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells.")
![Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human oral mucosa shows strong nuclear positivity in epithelial cells.](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Immunohistochemistry-NBP2-61621-img0003.jpg "Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human oral mucosa shows strong nuclear positivity in epithelial cells.")
![Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human small intestine shows moderate nuclear immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Immunohistochemistry-NBP2-61621-img0004.jpg "Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human small intestine shows moderate nuclear immunoreactivity in glandular epithelium cells.")
![Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human kidney shows weak to moderate nuclear positivity in a subset of cells.](http://images.novusbio.com/fullsize/TLE3-Antibody-CL3573-Immunohistochemistry-Paraffin-NBP2-61621-img0005.jpg "Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human kidney shows weak to moderate nuclear positivity in a subset of cells.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")