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TIMP-1 Antibody [Unconjugated]

 (22 Publications)
 (1 Review)
 
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Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by ...read more
Western blot shows lysates of PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 5 µg/mL of Goat ...read more
TIMP‑1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) at 1 µg/mL for 1 ...read more
Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ...read more
Genetic Strategies: Western blot shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line and TIMP-1 knockout SK-OV-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TIMP-1 ...read more
Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat ...read more
IHC staining result investigating TR4 level and macrophage infiltration in tumor tissues of PCa patients, as well as TIMP-1/MMP2/MMP9 signaling. IHC staining was performed using TR4 antibody (1:300) ,CD68 antibody ...read more
Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) ...read more
Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, B/N, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF970
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF970-SP
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TIMP-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # Q6FGX5
Specificity
Detects human TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TIMP‑1 and recombinant rat TIMP-1 is observed and less than 1% cross-reactivity with recombinant human (rh) TIMP-2, rhTIMP-3, and rhTIMP‑4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIMP1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Knockout Validated
  • Neutralization 1 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1-5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF970 in the following applications:
Publications
Read Publications using
AF970 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-1 Antibody [Unconjugated]

  • CLGI
  • Collagenase inhibitor
  • collagenase inhibitor)
  • EPATIMP-1
  • EPO
  • erythroid potentiating activity
  • Erythroid-potentiating activity
  • Fibroblast collagenase inhibitor
  • FLJ90373
  • HCI
  • metalloproteinase inhibitor 1
  • TIMP metallopeptidase inhibitor 1
  • TIMP1
  • TIMP-1
  • TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
  • Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TIMP-1 Antibody
[Unconjugated]
AF970
TIMP-1 Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC, B/N, KO

Publications for TIMP-1 Antibody (AF970)(22)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: IHC, IHC-P, Immunohistochemistry, In Vivo, Neutralization, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
IHC
(2)
IHC-P
(1)
Immunohistochemistry
(1)
In Vivo
(1)
Neutralization
(4)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(16)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF970 Applications Species
Wittig, F;Henkel, L;Prüser, J;Merkord, J;Ramer, R;Hinz, B; Inhibition of Monoacylglycerol Lipase Decreases Angiogenic Features of Endothelial Cells via Release of Tissue Inhibitor of Metalloproteinase-1 from Lung Cancer Cells Cells 2023-01-01 [PMID: 37443791] (Immunohistochemistry, Human) Immunohistochemistry Human
R Ishihara, T Oda, Y Murakami, I Matsumura, S Watanabe, Y Asao, Y Masuda, N Gotoh, T Kasamatsu, H Takei, N Kobayashi, N Sasaki, T Saitoh, H Murakami, H Handa Myeloma Microenvironmental TIMP1 Induces the Invasive Phenotype in Fibroblasts to Modulate Disease Progression International Journal of Molecular Sciences, 2023-01-22;24(3):. 2023-01-22 [PMID: 36768545] (Neutralization, Human) Neutralization Human
H Kawai, Y Bouchekiou, N Nishitani, K Niitani, S Izumi, H Morishita, C Andoh, Y Nagai, M Koda, M Hagiwara, K Toda, H Shirakawa, K Nagayasu, Y Ohmura, M Kondo, K Kaneda, M Yoshioka, S Kaneko Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion Nature Communications, 2022-12-22;13(1):7708. 12/22/2022 [PMID: 36550097]
Gerwinn T, Salemi S, Schori LJ et al. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction Frontiers in Cell and Developmental Biology 2022-10-04 [PMID: 36268506]
G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin Single cell transcriptomic landscape of diabetic foot ulcers Nature Communications, 2022-01-10;13(1):181. 2022-01-10 [PMID: 35013299] (IHC, Human) IHC Human
Y Kobayashi, K Tokuda, C Yamashiro, F Higashijim, T Yoshimoto, M Ota, T Ogata, A Ashimori, M Hatano, M Kobayashi, SH Uchi, M Wakuta, K Kimura Inhibition of epithelial-mesenchymal transition in retinal pigment epithelial cells by a retinoic acid receptor-&amp;alpha agonist Scientific Reports, 2021-06-04;11(1):11842. 2021-06-04 [PMID: 34088917] (Western Blot, Human) Western Blot Human
Vivian C. Chioma, Anna Kruyer, Ana-Clara Bobadilla, Ariana Angelis, Zachary Ellison, Ritchy Hodebourg et al. Heroin Seeking and Extinction From Seeking Activate Matrix Metalloproteinases at Synapses on Distinct Subpopulations of Accumbens Cells Biological Psychiatry 2021-05-01 [PMID: 33579535]
X Li, N Niu, J Sun, Y Mou, X He, L Mei IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1 FEBS Open Bio, 2020-11-09;0(0):. 2020-11-09 [PMID: 33064893] (Neutralization, Human) Neutralization Human
S Taylor, M Whitfield, J Barratt, A Didangelos The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix J. Immunol., 2020-09-11;0(0):. 2020-09-11 [PMID: 32917786] (IHC, Human) IHC Human
Barbar L, Jain T, Zimmer M et al. CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes Neuron 5/22/2020 [PMID: 32485136]
Show All 22 Publications.

Review for TIMP-1 Antibody (AF970) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF970:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen TIMP-1 AF970
Enlarge 		4
reviewed by:
Verified Customer
 IHC-Fr Human 07/18/2019
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
LotBUO0418071

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Immunohistochemistry-Frozen TIMP-1 AF970
Enlarge
 Verified Customer
07/18/2019
Application: IHC-Fr
Species: Human
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Bioinformatics

Gene Symbol TIMP1
Uniprot