![Western Blot: TGF beta Receptor III Overexpression Lysate (Adult Normal) [NBL1-16858] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TGF beta Receptor III.](http://images.novusbio.com/fullsize/TGF-beta-Receptor-III-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16858-img0002.jpg "Western Blot: TGF beta Receptor III Overexpression Lysate (Adult Normal) [NBL1-16858] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TGF beta Receptor III.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | TGF-beta RIII Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC216595 Accession#: NM_003243 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | TGFBR3 |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 93.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
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Research Areas for TGF-beta RIII Lysate (NBL1-16858)Find related products by research area.
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TGF-beta RIII - a high affinity reservoir for TGF-beta I/II ligands with therapeutic potential TGF beta (transforming growth factor beta) is a superfamily of cytokines that participate in a variety of cellular processes including growth, proliferation, differentiation, and apoptosis. There are 3 classes of receptors for TGF beta cytokines an... Read full blog post. |
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TGF-beta RIII - A multi-functional regulator of the TGF-beta signaling pathway Transforming growth factor-beta receptor III (TGF-beta RIII) is one of three receptors for the secreted growth factor TGF-beta. Unlike type I and type II TGF-beta receptors, TGF-beta RIII does not participate directly in the propagation of intracel... Read full blog post. |
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