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TDP-43/TARDBP Antibody (JM51-10)

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Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded human pancreas tissue usingTDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP-43/TARDBP antibody. The section was pre-treated using ...read more
Flow Cytometry: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of Hela cells with TDP-43/TARDBP Antibody (JM51-10) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with ...read more
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded mouse placenta tissue using TDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, Rt, ZeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
JM51-10
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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TDP-43/TARDBP Antibody (JM51-10) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Synthetic peptide within Human TDP-43/TARDBP aa 354-390 / 414; (2)aa 253-290 / 414. (SwissProt: Q13148 Human; SwissProt: Q921F2 Mouse; Unigene:2633 Rat; Entrez Gene:325052 Zebrafish)
Localization
Cytoplasm, Mitochondrion, Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TARDBP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation Use at an assay dependent concentration.
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for TDP-43/TARDBP Antibody (JM51-10)

  • ALS10
  • ALS10TAR DNA-binding protein-43
  • TAR DNA binding protein
  • TARDBP
  • TDP43
  • TDP-43
  • TDP-43TAR DNA-binding protein 43

Background

TARDBP, also known as TAR DNA binding protein, is a DNA and RNA-binding protein which regulates transcription and splicing. TARDBP is also involved in the regulation of CFTR splicing and promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. TARD-BP may also be involved in microRNA biogenesis, apoptosis and cell division. TARDBP can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TARDBP