Name: Progranulin/PGRN Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF2557
Rating: 4 (1 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	WB, IHC, ICC/IF
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF2557

Catalog# & Formulation	Size	Price
Carrier Free AF2557-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2557

Progranulin/PGRN Antibody [Unconjugated]

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Progranulin/PGRN Antibody [Unconjugated] Summary

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Publications for Progranulin/PGRN Antibody (AF2557)(55)

Review for Progranulin/PGRN Antibody (AF2557) (1) 41

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Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Progranulin Thr18-Leu589 Accession # P28798
Specificity	Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	Grn
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF2557	Applications	Species
Takahashi, H;Bhagwagar, S;Nies, SH;Ye, H;Han, X;Chiasseu, MT;Wang, G;Mackenzie, IR;Strittmatter, SM; Reduced progranulin increases tau and ?-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase Nature communications 2024-02-16 [PMID: 38365772] (IHC, Mouse)	IHC	Mouse
He, Y;Kaya, I;Shariatgorji, R;Lundkvist, J;Wahlberg, LU;Nilsson, A;Mamula, D;Kehr, J;Zareba-Paslawska, J;Biverstål, H;Chergui, K;Zhang, X;Andren, PE;Svenningsson, P; Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents Nature communications 2023-09-19 [PMID: 37726325] (IHC, Mouse)	IHC	Mouse
Feng T, Minevich G, Liu P et al. AAV-GRN partially corrects motor deficits and ALS/FTLD-related pathology in Tmem106bGrn mice iScience 2023-06-01 [PMID: 37519899]
Feng T, Minevich G, Liu P et al. AAV-GRN partially corrects motor deficits and ALS/FTLD-related pathology in Tmem106bGrn mice iScience 6/1/2023 [PMID: 37519899]
Anderson, A;Tansey, MG; Loss of Progranulin Results in Increased Pan-Cathepsin Activity and Reduced LAMP1 Lysosomal Protein bioRxiv : the preprint server for biology 2023-07-16 [PMID: 37503267] (Western Blot, Mouse)	Western Blot	Mouse
Zhang T, Feng T, Wu K et al. Progranulin deficiency results in sex-dependent alterations in microglia in response to demyelination Acta Neuropathologica 7/1/2023 [PMID: 37120788]
Zhang T, Feng T, Wu K et al. Progranulin deficiency results in sex-dependent alterations in microglia in response to demyelination Acta Neuropathologica 2023-07-01 [PMID: 37120788]
H Du, C Yang, AL Nana, WW Seeley, M Smolka, F Hu Progranulin inhibits phospholipase sPLA2-IIA to control neuroinflammation bioRxiv : the preprint server for biology, 2023-04-06;0(0):. 4/6/2023 [PMID: 37066328]
DM Smith, ML Niehoff, K Ling, P Jafar-Neja, F Rigo, SA Farr, MF Wilkinson, AD Nguyen Targeting nonsense-mediated RNA decay does not increase progranulin levels in the Grn R493X mouse model of frontotemporal dementia PLoS ONE, 2023-03-09;18(3):e0282822. 3/9/2023 [PMID: 36893203]
Y He, X Zhang, I Flais, P Svenningss Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia Pathophysiology International Journal of Molecular Sciences, 2022-10-10;23(19):. 2022-10-10 [PMID: 36233357] (IHC, Mouse)	IHC	Mouse
MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis The Journal of Biological Chemistry, 2022-08-04;0(0):102348. 2022-08-04 [PMID: 35933009] (Western Blot, Mouse)	Western Blot	Mouse
C Dedert, V Mishra, G Aggarwal, AD Nguyen, F Xu Progranulin Preserves Autophagy Flux and Mitochondrial Function in Rat Cortical Neurons Under High Glucose Stress Frontiers in Cellular Neuroscience, 2022-07-08;16(0):874258. 2022-07-08 [PMID: 35880011] (ICC, Western Blot, Rat)	ICC, Western Blot	Rat
Du H, Zhou X, Feng T, Hu F Regulation of lysosomal trafficking of progranulin by sortilin and prosaposin Brain Communications 1/4/2022 [PMID: 35169707]
T Zhang, H Du, MN Santos, X Wu, MD Pagan, LJ Trigiani, N Nishimura, T Reinheckel, F Hu Differential regulation of progranulin derived granulin peptides Molecular Neurodegeneration, 2022-02-04;17(1):15. 2022-02-04 [PMID: 35120524] (Western Blot, Mouse)	Western Blot	Mouse
Swathi Devireddy, Shawn M. Ferguson Efficient progranulin exit from the ER requires its interaction with prosaposin, a Surf4 cargo Journal of Cell Biology 2/7/2022 [PMID: 34919127]
Kei Takahashi, Shinsuke Nakamura, Masamitsu Shimazawa, Hideaki Hara Retinal Degeneration and Microglial Dynamics in Mature Progranulin-Deficient Mice International Journal of Molecular Sciences 10/26/2021 [PMID: 34768987]
Tingting Dong, Leon Tejwani, Youngseob Jung, Hiroshi Kokubu, Kimberly Luttik, Terri M. Driessen et al. Microglia regulate brain progranulin levels through the endocytosis/lysosomal pathway JCI Insight 11/22/2021 [PMID: 34618685]
Emilia A. Zin, Daisy Han, Jennifer Tran, Nikolas Morrison-Welch, Meike Visel, Mervi Kuronen et al. Outcomes of progranulin gene therapy in the retina are dependent on time and route of delivery Molecular Therapy - Methods & Clinical Development 9/10/2021 [PMID: 34485593]
K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation Journal of Neuroinflammation, 2021-07-25;18(1):164. 2021-07-25 [PMID: 34304733] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
Du H, Wong MY, Zhang T et al. A multifaceted role of progranulin in regulating amyloid-beta dynamics and responses Life science alliance 7/1/2021 [PMID: 34103390]
J Frew, HB Nygaard Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice Acta neuropathologica communications, 2021-04-01;9(1):57. 2021-04-01 [PMID: 33795008] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
M Murakoshi, T Gohda, E Adachi, S Ichikawa, S Hagiwara, Y Suzuki Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice Scientific Reports, 2021-01-13;11(1):1194. 2021-01-13 [PMID: 33441916] (IHC, Mouse)	IHC	Mouse
M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations Acta Neuropathol Commun, 2020-10-07;8(1):163. 10/7/2020 [PMID: 33028409]
Yungang Lan, Zi Li, Zhenzhen Wang, Xinran Wang, Gaili Wang, Jing Zhang et al. An Experimental Model of Neurodegenerative Disease Based on Porcine Hemagglutinating Encephalomyelitis Virus–Related Lysosomal Abnormalities Molecular Neurobiology 12/1/2020 [PMID: 32876841]
Feng T, Mai S, Roscoe JM et al. Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in mice EMBO Rep. 8/10/2020 [PMID: 32852886]
T Sasaki, M Shimazawa, H Kanamori, Y Yamada, A Nishinaka, Y Kuse, G Suzuki, T Masuda, S Nakamura, M Hosokawa, S Minatoguch, H Hara Effects of progranulin on the pathological conditions in experimental myocardial infarction model Sci Rep, 2020-07-16;10(1):11842. 2020-07-16 [PMID: 32678228] (IF, Western Blot, Mouse)	IF, Western Blot	Mouse
Kuang L, Hashimoto K, Huang EJ et al. Frontotemporal dementia nonsense mutation of progranulin rescued by aminoglycosides Hum. Mol. Genet. 1/8/2020 [PMID: 31913476]
FL Tang, L Zhao, Y Zhao, D Sun, XJ Zhu, L Mei, WC Xiong Coupling of terminal differentiation deficit with neurodegenerative pathology in Vps35-deficient pyramidal neurons Cell Death Differ., 2020-01-06;0(0):. 2020-01-06 [PMID: 31907392] (IHC, Mouse)	IHC	Mouse
C Wang, L Zhang, JC Ndong, A Hettinghou, G Sun, C Chen, C Zhang, R Liu, CJ Liu Progranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice J Neuroinflammation, 2019-11-27;16(1):238. 2019-11-27 [PMID: 31775776] (Western Blot, Mouse)	Western Blot	Mouse
F Wei, Z Jiang, H Sun, J Pu, Y Sun, M Wang, Q Tong, Y Bi, X Ma, GF Gao, J Liu Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling PLoS Pathog., 2019-10-04;15(10):e1008062. 2019-10-04 [PMID: 31585000] (In Vivo, Mouse)	In Vivo	Mouse
X Zhou, DH Paushter, MD Pagan, D Kim, M Nunez Sant, RL Lieberman, HS Overkleeft, Y Sun, MB Smolka, F Hu Progranulin deficiency leads to reduced glucocerebrosidase activity PLoS ONE, 2019-07-10;14(7):e0212382. 2019-07-10 [PMID: 31291241] (Western Blot, Mouse)	Western Blot	Mouse
LP Elia, AR Mason, A Alijagic, S Finkbeiner Genetic Regulation of Neuronal Progranulin Reveals a Critical Role for the Autophagy-Lysosome Pathway J. Neurosci., 2019-01-29;0(0):. 2019-01-29 [PMID: 30696728] (ELISA Development (Detection), Mouse)	ELISA Development (Detection)	Mouse
Shivapathasundram G, Wickremesekera A, Brasch H et al. SMCR8 negatively regulates AKT and MTORC1 signaling to modulate lysosome biogenesis and tissue homeostasis Autophagy 5/1/2019 [PMID: 30696333]
Andrew E. Arrant, Anthony J. Filiano, Aashka R. Patel, Madelyn Q. Hoffmann, Nicholas R. Boyle, Shreya N. Kashyap et al. Reduction of microglial progranulin does not exacerbate pathology or behavioral deficits in neuronal progranulin-insufficient mice Neurobiology of Disease 4/1/2019 [PMID: 30448285]
Y Kuse, K Ohuchi, S Nakamura, H Hara, M Shimazawa Microglia increases the proliferation of retinal precursor cells during postnatal development Mol. Vis., 2018-07-30;24(0):536-545. 2018-07-30 [PMID: 30090016] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity Acta Neuropathol Commun, 2018-05-31;6(1):42. 2018-05-31 [PMID: 29855382] (Western Blot, Mouse)	Western Blot	Mouse
N Uesaka, M Abe, K Konno, M Yamazaki, K Sakoori, T Watanabe, TH Kao, T Mikuni, M Watanabe, K Sakimura, M Kano Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum Neuron, 2018-02-01;0(0):. 2018-02-01 [PMID: 29398357] (IHC, Mouse)	IHC	Mouse
K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology Nat Commun, 2018-01-30;9(1):433. 2018-01-30 [PMID: 29382817] (IHC, Mouse)	IHC	Mouse
AE Arrant, VC Onyilo, DE Unger, ED Roberson Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis J. Neurosci., 2018-01-29;0(0):. 2018-01-29 [PMID: 29378861] (IHC-Fr, Western Blot, Mouse)	IHC-Fr, Western Blot	Mouse
H Sugihara, K Miyaji, K Yamanouchi, T Matsuwaki, M Nishihara Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle J. Vet. Med. Sci., 2017-12-18;0(0):. 2017-12-18 [PMID: 29249750] (IHC, Mouse)	IHC	Mouse
JM Van Kampen, DG Kay Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease PLoS ONE, 2017-08-24;12(8):e0182896. 2017-08-24 [PMID: 28837568] (IHC-Fr, Western Blot, Mouse)	IHC-Fr, Western Blot	Mouse
X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu Lysosomal processing of progranulin Mol Neurodegener, 2017-08-23;12(1):62. 2017-08-23 [PMID: 28835281] (Western Blot, Mouse)	Western Blot	Mouse
TL Petkau, J Blanco, BR Leavitt Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice Neurobiol. Dis., 2017-06-21;106(0):14-22. 2017-06-21 [PMID: 28647554] (IHC, Mouse)	IHC	Mouse
S Beel, M Moisse, M Damme, L De Muynck, W Robberecht, L Van Den Bo, P Saftig, P Van Damme Progranulin functions as a cathepsin D chaperone to stimulate axonal outgrowth in vivo Hum. Mol. Genet., 2017-08-01;26(15):2850-2863. 2017-08-01 [PMID: 28453791] (ICC, Mouse)	ICC	Mouse
Li Luo, Kaihua Guo, Wenguo Fan, Yinghong Lu, Lizhi Chen, Yang Wang et al. Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats Experimental and Therapeutic Medicine 2/1/2017 [PMID: 28352345]
Jinlong Jian, Qing-Yun Tian, Aubryanna Hettinghouse, Shuai Zhao, Helen Liu, Jianlu Wei et al. Progranulin Recruits HSP70 to beta -Glucocerebrosidase and Is Therapeutic Against Gaucher Disease EBioMedicine 11/1/2016 [PMID: 27789271]
Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina Sci Rep, 2016-03-31;6(0):23811. 2016-03-31 [PMID: 27030285] (IHC-Fr, Western Blot, Mouse)	IHC-Fr, Western Blot	Mouse
Andrew E. Arrant, Aashka R. Patel, Erik D. Roberson Effects of Exercise on Progranulin Levels and Gliosis in Progranulin-Insufficient Mice1,2,3 eNeuro 9/12/2015 [PMID: 26361634]
S. Sakura Minami, Sang-Won Min, Grietje Krabbe, Chao Wang, Yungui Zhou, Rustam Asgarov et al. Progranulin protects against amyloid beta deposition and toxicity in Alzheimer's disease mouse models Nature Medicine 10/1/2014 [PMID: 25261995]
Brian P. Hafler, Zoe A. Klein, Z. Jimmy Jimmy Zhou, Stephen M. Strittmatter Progressive retinal degeneration and accumulation of autofluorescent lipopigments in Progranulin deficient mice Brain Research 11/7/2014 [PMID: 25234724]
Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells. Mol Med Rep, 2014-03-06;9(5):1675-80. 2014-03-06 [PMID: 24604062] (ICC, Western Blot, Mouse)	ICC, Western Blot	Mouse
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts. Biochem Biophys Res Commun, 2012-12-01;430(1):419-24. 2012-12-01 [PMID: 23206705] (Western Blot, Mouse)	Western Blot	Mouse
Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L Progranulin regulates neuronal outgrowth independent of sortilin. Mol Neurodegener, 2012-07-10;7(0):33. 2012-07-10 [PMID: 22781549] (Western Blot, Mouse)	Western Blot	Mouse
Almeida S, Zhou L, Gao FB Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins. PLoS ONE, 2011-10-18;6(10):e26454. 2011-10-18 [PMID: 22028881] (ICC, Mouse)	ICC	Mouse
Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models. J. Pathol., 2009-10-01;219(2):173-81. 2009-10-01 [PMID: 19557827] (IHC-P, Western Blot, Mouse)	IHC-P, Western Blot	Mouse
Show All 55 Publications. Collapse Publications.

	Verified Customer 03/19/2018
Application:	Immunohistochemistry with free floating section
Species:	Mouse

Gene Symbol	Grn
Uniprot	P28798(Mouse)