Home » TBK1 » TBK1 Antibodies » TBK1 Antibody (108A429) [mFluor Violet 450 SE]
Novus Biologicals products are now on bio-techne.com

TBK1 Antibody (108A429) [mFluor Violet 450 SE]

 
 Submit a Review

Images

 
There are currently no images for TBK1 Antibody (NB100-56705MFV450).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP, KO
Clone
108A429
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 450 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56705AF350
Alexa Fluor 405
NB100-56705AF405
Alexa Fluor 488
NB100-56705AF488
Alexa Fluor 532
NB100-56705AF532
Alexa Fluor 594
NB100-56705AF594
Alexa Fluor 647
NB100-56705AF647
Alexa Fluor 700
NB100-56705AF700
Alexa Fluor 750
NB100-56705AF750
Biotin
NB100-56705B
CoraFluor 1
NB100-56705CL1
DyLight 350
NB100-56705UV
DyLight 405
NB100-56705V
DyLight 488
NB100-56705G
DyLight 550
NB100-56705R
DyLight 594
NB100-56705DL594
DyLight 650
NB100-56705C
DyLight 680
NB100-56705FR
DyLight 755
NB100-56705IR
FITC
NB100-56705F
HRP
NB100-56705H
Janelia Fluor 525
NB100-56705JF525
Janelia Fluor 549
NB100-56705JF549
Janelia Fluor 635
NB100-56705JF635
Janelia Fluor 646
NB100-56705JF646
Janelia Fluor 669
NB100-56705JF669
Unconjugated
NB100-56705
mFluor Violet 500 SE
NB100-56705MFV500
mFluor Violet 610 SE
NB100-56705MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56705
BSA Free
NB100-56705SS
Azide and BSA Free
NBP2-33243
BSA Free
NB100-56705
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

TBK1 Antibody (108A429) [mFluor Violet 450 SE] Summary

Immunogen
A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TBK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TBK1 Antibody (108A429) [mFluor Violet 450 SE]

  • EC 2.7.11
  • EC 2.7.11.1
  • FLJ11330
  • FTDALS4
  • NAK serine/threonine-protein kinase TBK 1
  • NAK
  • NAKserine/threonine-protein kinase TBK1
  • NF-kappa-B-activating kinase
  • NFKB-Activating Kinase
  • T2K
  • TANK-binding kinase 1NF-kB-activating kinase
  • TBK 1
  • TBK1

Background

NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

IRF3 Antibody
(SD2062)
NBP2-67741
Immunocytochemistry/ Immunofluorescence IRF3 Antibody (SD2062)
Immunocytochemistry/ Immunofluorescence IRF3 Antibody (SD2062)
Immunohistochemistry-Paraffin IRF3 Antibody (SD2062)
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TRIF/TICAM1
Antibody - BSA Free
NB120-13810
Knockdown Validated TRIF/TICAM1 Antibody - BSA Free
Unknown TRIF/TICAM1 Antibody - BSA Free
Immunohistochemistry-Paraffin TRIF/TICAM1 Antibody - BSA Free
(37 Publications)
Species: Mu, Po, Rt
Applications: IB, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
MAVS Antibody -
BSA Free
NB100-80859
Immunocytochemistry/ Immunofluorescence MAVS Antibody - BSA Free
Western Blot MAVS Antibody - BSA Free
Immunohistochemistry-Paraffin MAVS Antibody - BSA Free
(1 Review)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
TLR4 Antibody
(76B357.1) - BSA Free
NB100-56566
Dual RNAscope ISH-IHC TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
(5 Reviews)
(141 Publications)
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
TLR3 Antibody
(40C1285.6) - BSA Fre
NBP2-24875
Flow Cytometry TLR3 Antibody (40C1285.6) - BSA Free
Western Blot TLR3 Antibody (40C1285.6) - BSA Free
Flow Cytometry TLR3 Antibody (40C1285.6) - BSA Free
(87 Publications)
Species: Ca, Hu, Mu
Applications: BA, B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-P, WB
RIG-I Antibody
AF4859
Western Blot RIG-I Antibody
(2 Publications)
Species: Hu
Applications: WB
IKK epsilon/IKBKE
Antibody (346909) [Un ...
MAB3199
Immunocytochemistry IKK epsilon/IKBKE Antibody (346909) [Unconjugated]
Western Blot IKK epsilon/IKBKE Antibody (346909) [Unconjugated]
Immunocytochemistry IKK epsilon/IKBKE Antibody (346909) [Unconjugated]
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(817 Publications)
Species: Hu
Applications: BA
IFN-beta
[Unconjugated]
8499-IF
Binding Activity IFN-beta [Unconjugated]
(52 Publications)
Species: Hu
Applications: BA
RNF36 Antibody
NB100-1207
Immunohistochemistry-Paraffin RNF36 Antibody
Immunohistochemistry-Paraffin RNF36 Antibody
Western Blot RNF36 Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, PEP-ELISA, WB
IRF7 Antibody
(SC0617)
NBP2-67634
Immunocytochemistry/ Immunofluorescence IRF7 Antibody (SC0617)
Immunocytochemistry/ Immunofluorescence IRF7 Antibody (SC0617)
Immunohistochemistry-Paraffin IRF7 Antibody (SC0617)
(1 Publication)
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
TANK Antibody
NB100-56144
Immunohistochemistry TANK Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
STING/TMEM173
Antibody (723505) [Un ...
MAB7169
Intracellular Staining by Flow Cytometry STING/TMEM173 Antibody (723505) [Unconjugated]
Intracellular Staining by Flow Cytometry STING/TMEM173 Antibody (723505) [Unconjugated]
Intracellular Staining by Flow Cytometry STING/TMEM173 Antibody (723505) [Unconjugated]
(1 Review)
(28 Publications)
Species: Hu
Applications: CyTOF-ready, ICC, IP, ICFlow, WB
IKK beta Antibody
(10AG2) - BSA Free
NB100-56509
Flow Cytometry IKK beta Antibody (10AG2) - BSA Free
Immunocytochemistry/ Immunofluorescence IKK beta Antibody (10AG2) - BSA Free
Flow Cytometry IKK beta Antibody (10AG2) - BSA Free
(2 Reviews)
(60 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, IB, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
IKK alpha Antibody
(14A231) - BSA Free
NB100-56704
Immunocytochemistry/ Immunofluorescence IKK alpha Antibody (14A231) - BSA Free
Immunocytochemistry/ Immunofluorescence IKK alpha Antibody (14A231) - BSA Free
Immunohistochemistry-Paraffin IKK alpha Antibody (14A231) - BSA Free
(85 Publications)
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
TRAF-3 Antibody
NB100-56176
Immunohistochemistry-Paraffin TRAF-3 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
MyD88 Inhibitor
Peptide Set
NBP2-29328
MyD88 Inhibitor Peptide Set
(3 Reviews)
(99 Publications)
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo

Publications for TBK1 Antibody (NB100-56705MFV450) (0)

There are no publications for TBK1 Antibody (NB100-56705MFV450).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TBK1 Antibody (NB100-56705MFV450) (0)

There are no reviews for TBK1 Antibody (NB100-56705MFV450). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TBK1 Antibody (NB100-56705MFV450). (Showing 1 - 6 of 6 FAQ).

  1. What is the exact sequence this TBK1 antibody recognizes?
    • The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
  2. I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
    • While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
  3. We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
    • Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
  4. Is there a smaller size of your TBK1 antibody?
    • This TBK1 antibody is offered in a smaller 0.025 mg size.
  5. Are there any publications where this TBK1 antibody was used in western blot for mouse?
    • This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
  6. Cant this TBK1 antibody be conjugated to PE for FLOW?
    • Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

Secondary Antibodies

 

Isotype Controls

Additional TBK1 Products

Research Areas for TBK1 Antibody (NB100-56705MFV450)

Find related products by research area.

Blogs on TBK1.

Check out the latest blog posts on TBK1.
Autophagy Research Update: What a difference a year makes!
By Christina Towers, PhD Over the last two decades the field of autophagy has exploded! Innovative techniques, comprehensive analysis and disease-relevant models have yielded basic and clinical discoveries of conseque...  Read full blog post.
STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Dual RNAscope ISH-IHC: TLR4 Antibody (76B357.1) [NB100-56566] - FFPE tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, ACD catalog # 311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog # NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.
TLR4 Antibody (76B357.1) - BSA Free
NB100-56566

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TBK1 Antibody (108A429) [mFluor Violet 450 SE] and receive a gift card or discount.


Bioinformatics

Gene Symbol TBK1