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![Knockout Validated: TBK1 Antibody (108A429) [NB100-56705] - Western blot using lysates from U20S parental cell line and TBK1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse/Rat TBK1 Monoclonal Antibody (Catalog # NB100-56705) at 1 ug/ul O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band at 83 kDa was detected for TBK1 in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Knockout-Validated-NB100-56705-img0023.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: TBK1 Antibody (108A429) [NB100-56705] - Western blot using lysates from U20S parental cell line and TBK1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse/Rat TBK1 Monoclonal Antibody (Catalog # NB100-56705) at 1 ug/ul O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band at 83 kDa was detected for TBK1 in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Simple Western: TBK1 Antibody (108A429) [NB100-56705] - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Simple-Western-NB100-56705-img0014.jpg)
![Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunohistochemistry-Paraffin-NB100-56705-img0021.jpg)
![Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Western-Blot-NB100-56705-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunocytochemistry-Immunofluorescence-NB100-56705-img0013.jpg)
![Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) Image from the Azide and BSA Free version of this antibody.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunohistochemistry-Paraffin-NB100-56705-img0022.jpg)
![Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Western-Blot-NB100-56705-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TBK1 at 20 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunocytochemistry-Immunofluorescence-NB100-56705-img0019.jpg)
![Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189] - Western blot analysis of extracts of various cell lines, using TBK1 Rabbit mAb (NBP3-16189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/TBK1-Antibody-4G6C7-Western-Blot-NBP3-16189-img0005.jpg "Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189] - Western blot analysis of extracts of various cell lines, using TBK1 Rabbit mAb (NBP3-16189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (4G6C7) [NBP3-16189] - Immunofluorescence analysis of U-2 OS cells using TBK1 Rabbit mAb (NBP3-16189) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TBK1-Antibody-4G6C7-Immunocytochemistry-Immunofluorescence-NBP3-16189-img0003.jpg)
![Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189] - Western blot analysis of extracts of various cell lines, using TBK1 Rabbit mAb (NBP3-16189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/TBK1-Antibody-4G6C7-Western-Blot-NBP3-16189-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (4G6C7) [NBP3-16189] - Immunofluorescence analysis of C6 cells using TBK1 Rabbit mAb (NBP3-16189) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TBK1-Antibody-4G6C7-Immunocytochemistry-Immunofluorescence-NBP3-16189-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (4G6C7) [NBP3-16189] - Immunofluorescence analysis of NIH-3T3 cells using TBK1 Rabbit mAb (NBP3-16189) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TBK1-Antibody-4G6C7-Immunocytochemistry-Immunofluorescence-NBP3-16189-img0002.jpg)
![Immunoprecipitation: TBK1 Antibody (4G6C7) [NBP3-16189] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug TBK1 antibody (NBP3-16189). Western blot was performed from the immunoprecipitate using TBK1 antibody (NBP3-16189) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TBK1-Antibody-4G6C7-Immunoprecipitation-NBP3-16189-img0006.jpg)
![Western Blot: TBK1 Antibody [NBP2-93921] - Analysis of extracts of Rat thymus, using TBK1/NAK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TBK1-Antibody-Western-Blot-NBP2-93921-img0003.jpg "Western Blot: TBK1 Antibody [NBP2-93921] - Analysis of extracts of Rat thymus, using TBK1/NAK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: TBK1 Antibody [NBP2-93921] - Rat testis using TBK1/NAK Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TBK1-Antibody-Immunohistochemistry-Paraffin-NBP2-93921-img0005.jpg)
![Western Blot: TBK1 Antibody [NBP2-93921] - Analysis of extracts of Mouse lung, using TBK1/NAK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TBK1-Antibody-Western-Blot-NBP2-93921-img0001.jpg)
![Western Blot: TBK1 Antibody [NBP2-93921] - Analysis of extracts of various cell lines, using TBK1/NAK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/TBK1-Antibody-Western-Blot-NBP2-93921-img0002.jpg)
![Immunohistochemistry-Paraffin: TBK1 Antibody [NBP2-93921] - Rat kidney using TBK1/NAK Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TBK1-Antibody-Immunohistochemistry-Paraffin-NBP2-93921-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody [NBP2-13416] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/TBK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13416-img0002.jpg "Immunocytochemistry/Immunofluorescence: TBK1 Antibody [NBP2-13416] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: TBK1 Antibody [NBP2-13416] - Staining of human testis shows moderate cytoplasmic, membranous and nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/TBK1-Antibody-Immunohistochemistry-Paraffin-NBP2-13416-img0001.jpg)
![Immunohistochemistry: TBK1 Antibody (14D10) [NBP3-26292] - Image of TBK1 Antibody (14D10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26292_rabbit-tbk1-mab-14d10-262202414225222.jpg "Immunohistochemistry: TBK1 Antibody (14D10) [NBP3-26292] - Image of TBK1 Antibody (14D10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: TBK1 Antibody (14D10) [NBP3-26292] - Image of TBK1 Antibody (14D10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26292_rabbit-tbk1-mab-14d10-27220248504610.jpg)
![Flow Cytometry: TBK1 Antibody (14D10) [NBP3-26292] - Overlay Peak curve showing A549 cells stained with TBK1 Antibody (14D10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26292_rabbit-tbk1-mab-14d10-282202414183618.jpg)
![Western Blot: TBK1 [p Ser 172] Antibody (SR2251) [NBP3-21592] - Western blot analysis using NBP3-21592 in (1) HeLa cell lysate; (2) HeLa cell treated with Calyculin A .](http://images.novusbio.com/fullsize/nbp3-21592_rabbit-tbk1-p-ser-172-mab-sr2251-57202317215195.jpg "Western Blot: TBK1 [p Ser 172] Antibody (SR2251) [NBP3-21592] - Western blot analysis using NBP3-21592 in (1) HeLa cell lysate; (2) HeLa cell treated with Calyculin A .")
![Western Blot: TBK1 Antibody (S01-1G3) [NBP3-19537] - Western blot detection of TBK1 in Jurkat, C6, CHO-K1, Hela cell lysates using NBP3-19537 (1:1000 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.](http://images.novusbio.com/fullsize/TBK1-Antibody-S01-1G3-Western-Blot-NBP3-19537-img0001.jpg "Western Blot: TBK1 Antibody (S01-1G3) [NBP3-19537] - Western blot detection of TBK1 in Jurkat, C6, CHO-K1, Hela cell lysates using NBP3-19537 (1:1000 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.")
![Western Blot: TBK1 [p Ser172] Antibody (5B8P1) [NBP3-16918] - Western blot analysis of extracts of various cell lines, using TBK1 Rabbit mAb (NBP3-16918) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/TBK1-p-Ser172-Antibody-5B8P1-Western-Blot-NBP3-16918-img0001.jpg "Western Blot: TBK1 [p Ser172] Antibody (5B8P1) [NBP3-16918] - Western blot analysis of extracts of various cell lines, using TBK1 Rabbit mAb (NBP3-16918) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody [NBP2-55777] - Staining of human cell line RT4 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/TBK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55777-img0001.jpg "Immunocytochemistry/Immunofluorescence: TBK1 Antibody [NBP2-55777] - Staining of human cell line RT4 shows localization to nucleoplasm.")
![Western Blot: NAK Overexpression Lysate (Adult Normal) [NBP2-10346] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAK.](http://images.novusbio.com/fullsize/NAK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10346-img0001.jpg "Western Blot: NAK Overexpression Lysate (Adult Normal) [NBP2-10346] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAK.")