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MRG15 Antibody

Images

 
Western Blot: MRG15 Antibody [NB100-1307] - staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: MRG15 Antibody [NB100-1307] - staining of Mouse Liver (A), Rat Liver (B) and Pig Liver (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunohistochemistry: Goat Polyclonal MRG15 Antibody [NB100-1307] - (5µg/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Control: Goat Polyclonal MRG15 Antibody [NB100-1307] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MRG15 Antibody Summary

Immunogen
Peptide with sequence APKQDPKPKFQEG-C corresponding to N-Terminus according to NP_006782.1, NP_996670.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_006782.1) and isoform 2 (NP_996670.1).
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MORF4L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4-6 ug/ml
  • Immunohistochemistry-Paraffin 4-6 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1-0.3 ug/ml
Application Notes
Western blot: Approx 37kDa band observed in nuclear lysates of cell lines HepG2 and NIH3T3 and approx 40kDa band observed in Mouse, Rat and Pig Liver lysates (calculated MW of 37.2kDa according to Human NP_006782.1, Mouse NP_077751.1, Rat NP_001011999.1 and Pig XP_001927776.1 and 41.5kDa according to Human NP_996670.1, Mouse NP_001034236.1 and Pig XP_001927789.1).
Control
MRG15 Overexpression Lysate
Publications
Read Publication using NB100-1307.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MRG15 Antibody

  • Eaf3
  • Esa1p-associated factor 3 homolog
  • HsT17725
  • MEAF3
  • MGC10631
  • MORF-related gene on chromosome 15
  • MORFRG15
  • mortality factor 4 like 1
  • mortality factor 4-like protein 1
  • MRG15MORF-related gene 15 protein
  • Protein MSL3-1
  • S863-6
  • Transcription factor-like protein MRG15

Background

MRG15 is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MRG15 Antibody (NB100-1307)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MRG15 Antibody (NB100-1307) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MORF4L1
Entrez
Uniprot