Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ on HEK 293 cell lysate using NB110-58359.
Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ (Green) in HepG2 cells using NB110-58359. Nuclei (Blue) are counterstained with Hoecsht 33258.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NB110-58359] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using TAZ/WWTR1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The ...read more
Simple Western: TAZ/WWTR1 Antibody [NB110-58359] - Simple Western lane view shows a specific band for TAZ in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Immunoprecipitation: TAZ/WWTR1 Antibody - BSA Free [NB110-58359] - TAZ knockout in muscle decreases mitochondrial mass, respiration, & exercise ability. b Proteins of gastrocnemius muscle of WT & mKO mice analysed via ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for TAZ/WWTR1 Antibody - BSA Free
FLJ27004
FLJ45718
TAZ
TAZDKFZp586I1419
transcriptional co-activator with PDZ-binding motif
Transcriptional coactivator with PDZ-binding motif
WW domain containing transcription regulator 1
WW domain-containing transcription regulator protein 1
WWTR1
Background
TAZ (transcriptional co-activator with PDZ-binding motif) was identified as a binding partner of 14-3-3 family of proteins which contains seven homologous proteins with ability to bind phosphorylated serine with certain sequence motifs, thereby involving in diverse cellular functions such as differentiation, cell cycle progression and apoptosis via their interaction with diverse intracellular phosphoproteins involved in signaling networks. TAZ is homologous to YAP and displays transcriptional co-activator function via interaction with PPXY-containing transcriptional factors through its WW domain. Several transcriptional factors such as Runx/PEBP2, AP2, C/EBP, c-Jun, Krox-20, Krox-24, MEF2B, NF-E2, Oct-4 and p73 containing proline-rich PPXY motif have been proposed as TAZ's interacting partners. TAZ is also a major target of the Hippo core kinase cascade which regulates organ size control as well as stem cell properties by governing cell proliferation and apoptosis processes. Hippo mediated phosphorylation of YAP and TAZ leads to their sequestration into the cytoplasm by interaction with 14-3-3 proteins and ubiquitination-dependent proteosomal degradation, thereby governing its distribution, protein levels and functionality. TAZ interacts primarily with transcriptional factors TEAD1-4 (TEADs) and activates expression of target genes such as CTGF, IGFBP3, ITGB2, Birc5/Survivin, Gli2, and Axl.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TAZ/WWTR1 Antibody (NB110-58359). (Showing 1 - 4 of 4 FAQs).
I want to know which TAZ primary antibody is best produced for WB (human and mouse)!
I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.
We have an inquiry for your product #NB110-58359, TAZ Antibody. Our customer would like to try this product with rat cells lysate. Could you please let me know if you have data image of rat cells lysate can provide?
This antibody has been validated and is therefore guaranteed for use in human, rat and mouse in the following applications: Western blot, Immunoprecipitation and ICC/IF. We do not currently have a WB image that we can share however rat is one of the guaranteed species.
Our customer was interested in the TAZ Antibody (Cat.NB110-58359). Could you please help to confirm if it has been tested with rat cells?
The TAZ antibody, NB110-58359, has been tested and validated for use in rat and is therefore guaranteed. Our 100% guarantee covers all of our products for the listed species and applications.
I am looking for a WWTR1 antibody and NB110-58359 looks like the best one. I wonder if you guys can provide me the target sequence information of the antibody?
WWTR1 antibody (NB110-58359) is generated against residues 178-197 of WWTR1 (immunogen accession number Q9GZV5). The exact immunogen sequence for this antibody is: CPQRSMAVSQPNLVMNHQHQQ
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![Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ on HEK 293 cell lysate using NB110-58359.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Western-Blot-NB110-58359-img0006.jpg "Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ on HEK 293 cell lysate using NB110-58359.")
![Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ (Green) in HepG2 cells using NB110-58359. Nuclei (Blue) are counterstained with Hoecsht 33258.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58359-img0005.jpg "Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ (Green) in HepG2 cells using NB110-58359. Nuclei (Blue) are counterstained with Hoecsht 33258.")
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NB110-58359] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using TAZ/WWTR1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear and cytoplasmic staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NB110-58359-img0010.jpg "Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NB110-58359] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using TAZ/WWTR1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear and cytoplasmic staining was observed. Staining was performed by Histowiz.")
![Simple Western: TAZ/WWTR1 Antibody [NB110-58359] - Simple Western lane view shows a specific band for TAZ in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Simple-Western-NB110-58359-img0008.jpg "Simple Western: TAZ/WWTR1 Antibody [NB110-58359] - Simple Western lane view shows a specific band for TAZ in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunoprecipitation: TAZ/WWTR1 Antibody - BSA Free [NB110-58359] - TAZ knockout in muscle decreases mitochondrial mass, respiration, & exercise ability. b Proteins of gastrocnemius muscle of WT & mKO mice analysed via immunoblotting for detection of mitochondrial marker proteins. Alpha-tubulin used as loading control. Representative data shown & experiment performed twice w/ similar results. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/35115527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58359_rabbit-polyclonal-taz-wwtr1-antibody-3102024153977.jpg "Immunoprecipitation: TAZ/WWTR1 Antibody - BSA Free [NB110-58359] - TAZ knockout in muscle decreases mitochondrial mass, respiration, & exercise ability. b Proteins of gastrocnemius muscle of WT & mKO mice analysed via immunoblotting for detection of mitochondrial marker proteins. Alpha-tubulin used as loading control. Representative data shown & experiment performed twice w/ similar results. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/35115527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
