Orthogonal Strategies: Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Analysis in human cerebral cortex and liver tissues. Corresponding STMN3 RNA-seq data are presented for the same tissues.
Western Blot: STMN3 Antibody [NBP1-80779] - Analysis in control (vector only transfected HEK293T lysate) and STMN3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Staining of human liver shows no positivity in hepatocytes.
Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Staining of human testis shows no positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: STMN3 Antibody [NBP1-80779] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
This antibody was developed against a recombinant protein corresponding to the amino acids: TQEAQVLKQLAERREHEREVLHKALEENNNFSRQAEEKLNYKMELSKEIREAHLAALRERLREKELHAAEVRRNKEQREEMSG
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STMN3
Purity
Immunogen affinity purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for STMN3 Antibody
SCG10-like-protein
SCLIPSCG10-like protein
stathmin-3
stathmin-like 3
Background
The protein encoded by the STMN3 gene belongs to the stathmin/oncoprotein 18 family of microtubule-destabilizing phosphoproteins. It is similar to the SCG10 protein and is involved in signal transduction and regulation of microtubule dynamics. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for STMN3 Antibody (NBP1-80779). (Showing 1 - 4 of 4 FAQs).
Are there any reference publication used to obtain the immunogen sequence/any publication that generated the antibody with the same immunogen sequence?
The sequence comes from the consensus sequence on UniProt Q9NZ72 (https://www.uniprot.org/uniprotkb/Q9NZ72/entry)
Was the antibody tested on breast cancer cell lines before?
The only Western blot testing data is shown on our datasheet.
Does the antibody detect STMN1, STMN2 or STMN4 as well?
This sequence shares 77% homology with STMN2 and 67% with STMN1 and 4 so is not predicted to cross-react.
Does the antibody pick up all STMN3 protein?
The predicted molecular weight of STMN3 is approximately 21 kDa. This protein also has multiple post-translational modifications (http://www.uniprot.org/uniprot/Q9NZ72). The lowest of the strong bands is STMN3 and we suspect the strong band just above it near the 28 kDa marker is the modified version but we haven't confirmed this.
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