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Stathmin-2/STMN2 Antibody

Images

 
Genetic Strategies: Analysis of endogenous STMN2 in mouse dorsal root ganglia (DRG) neurons. A partial siRNA knockdown was used in the second lane. NBP1-49461 was used at a dilution of 1:1000. Image courtesy of ...read more
Staining of STMN2 in primary mouse dorsal root ganglia (DRG) neurons Image shows the expected staining of endogenous STMN2 in axons and growth cones. Tubulin is a marker of axons. NBP1-49461 was used at a dilution of ...read more
Neuronal deletion of Tsc2 improves axon regeneration within the first 2 d following nerve injury. SCG10 immunostaining of sciatic nerves 1 d after crush injury in control and Tsc2 cKO mice with (conditioned) or without ...read more
Expression of Tagged Proteins: Staining of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:1000 (A, B) and 1:3000 (C). Image courtesy of Dr. Jung Eun Shin.
Expression of Tagged Proteins: Analysis of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:4000. Image courtesy of Dr. Jung Eun Shin.
Stathmin-2/STMN2 immunoreactivity at the trigeminal root entry zone (antibody diluted 1:1000, tissue taken from a mouse with experimental autoimmune encephalomyelitis). Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Func, ICC/IF, IHC, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Stathmin-2/STMN2 Antibody Summary

Immunogen
C-terminal peptide of mouse STMN2. [Swiss-Prot P55821]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STMN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry Whole-Mount reported in scientific literature (PMID 35042776)
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen 1:200 - 1:500
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 31182472)
  • In vitro assay reported in scientific literature (PMID 22726832)
  • In vivo assay reported in scientific literature (PMID 22726832)
  • Knockdown Validated
  • Western Blot 1 - 2 ug/ml
Application Notes
In Western blot a band can be seen at ~22 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-49461 in the following applications:

Publications
Read Publications using
NBP1-49461 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32778834).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Stathmin-2/STMN2 Antibody

  • FLJ34868
  • FLJ50995
  • neuron-specific growth-associated protein
  • Protein SCG10
  • SCG10
  • SCG10neuronal growth-associated protein (silencer element)
  • SCGN10
  • SCGN10stathmin-2
  • Stathmin2
  • Stathmin-2
  • stathmin-like 2
  • STMN2
  • superior cervical ganglia, neural specific 10
  • Superior cervical ganglion-10 protein

Background

STMN2 (Stathmin-2 or SCG10/Scgn10) belongs to stathmin family of proteins which are known to bind tubulin for acting as a sequestering agents and for promoting microtubule disassembly. STMN2 is a membrane-associated neuronal protein expressed mainly during development and its expression correlates with neurite outgrowth. STMN2 possesses a unique N-terminal domain that is critical for membrane binding, is responsible for STMN2 localization to the Golgi complex and is important in its targeting to growth cones. STMN2 localize to perinuclear cytoplasm, axons, and growth cones of developing neurons, and exists in soluble as well as membrane-bound forms. STMN2 interacts with ITMC2, as well as MAPK8, and when phosphorylated by MAPK8, it act as regulator of microtubule stability which controls neurite length in cortical neurons. Unlike other stathmins, STMN2 acts in two ways to promote microtubule dynamics: it stabilizes microtubules at their plus end and promotes microtubule catastrophe at their minus end. In the developing brain, STMN2 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Reduced STMN2 expression is associated with Down's syndrome and Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Stathmin-2/STMN2 Antibody (NBP1-49461)(86)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 13 applications: Block/Neutralize, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IHC-WhMt, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, In Vivo, WB, Western Blot.


Filter By Application
Block/Neutralize
(2)
ICC/IF
(16)
IF/IHC
(18)
IHC
(2)
IHC-Fr
(10)
IHC-P
(3)
IHC-WhMt
(1)
Immunocytochemistry/ Immunofluorescence
(4)
Immunohistochemistry
(4)
Immunohistochemistry-Frozen
(1)
In Vivo
(2)
WB
(4)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(35)
Rat
(9)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 86. Show All 86 Publications.
Publications using NBP1-49461 Applications Species
Deininger S, Schumacher J, Blechschmidt A et al. Nerve injury converts Schwann cells in a long-term repair-like state in human neuroma tissue. Experimental neurology 2024-10-01 [PMID: 39362479]
Aaron D. Talsma, Jon P. Niemi, Richard E. Zigmond Neither injury induced macrophages within the nerve, nor the environment created by Wallerian degeneration is necessary for enhanced in vivo axon regeneration after peripheral nerve injury Journal of Neuroinflammation 2024-05-27 [PMID: 38802868]
Matthew Grove, Hyukmin Kim, Shuhuan Pang, Jose Paz Amaya, Guoqing Hu, Jiliang Zhou, Michel Lemay, Young-Jin Son, Klaus-Armin Nave, Timothy E Behrens TEAD1 is crucial for developmental myelination, Remak bundles, and functional regeneration of peripheral nerves eLife 2024-03-08 [PMID: 38456457]
Jone López-Erauskin, Mariana Bravo-Hernandez, Maximiliano Presa, Michael W Baughn, Ze'ev Melamed, Melinda S Beccari, Ana Rita Agra de Almeida Quadros, Olatz Arnold-Garcia, Aamir Zuberi, Karen Ling, Oleksandr Platoshyn, Elkin Niño-Jara, I Sandra Ndayambaje, Melissa McAlonis-Downes, Larissa Cabrera, Jonathan W Artates, Jennifer Ryan, Anita Hermann, John Ravits, C Frank Bennett, Paymaan Jafar-Nejad, Frank Rigo, Martin Marsala, Cathleen M Lutz, Don W Cleveland, Clotilde Lagier-Tourenne Stathmin-2 loss leads to neurofilament-dependent axonal collapse driving motor and sensory denervation. Nature neuroscience 2024-01-10 [PMID: 37996528]
Wang Y, Halawani D, Estill M et al. Aryl hydrocarbon receptor restricts axon regeneration of DRG neurons in response to injury bioRxiv : the preprint server for biology 2023-11-05 [PMID: 37961567] (ICC/IF, Mouse) ICC/IF Mouse
De Virgiliis F, Mueller F, Palmisano I et al. The circadian clock time tunes axonal regeneration Cell metabolism 2023-11-03 [PMID: 37951214]
Chen Q, Zhang L, Zhang F, Yi S FOSL1 modulates Schwann cell responses in the wound microenvironment and regulates peripheral nerve regeneration The Journal of biological chemistry 2023-11-08 [PMID: 37949219] (IHC-Fr, Rat) IHC-Fr Rat
Gupta DP, Bhusal A, Rahman MH et al. EBP50 is a key molecule for the Schwann cell-axon interaction in peripheral nerves Progress in neurobiology 2023-11-07 [PMID: 37940033] (IHC-P, Mouse)

Details:
1:500 IHC-P dilution
IHC-P Mouse
Gonçalves TM, Stewart CL, Baxley SD et al. Towards a comprehensive regulatory map of Mammalian Genomes Research square 2023-09-28 [PMID: 37841836] (IHC-Fr, Chicken)

Details:
Fertilized White Leghorn chicken eggs
IHC-Fr Chicken
Jeon Y, Shin YK, Kim H et al. ?Pix Guanine Nucleotide Exchange Factor Regulates Regeneration of Injured Peripheral Axons Int J Mol Sci 2023-09-20 [PMID: 37762659] (Block/Neutralize) Block/Neutralize
Show All 86 Publications.

Reviews for Stathmin-2/STMN2 Antibody (NBP1-49461) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using NBP1-49461:
Filter by Applications
IHC-Fr
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Stathmin-2/STMN2 NBP1-49461
Enlarge
5
reviewed by:
Kevin Thorburn
IHC-Fr Mouse 11/29/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse trigeminal root entry zone,trigeminal root entry zone
SpeciesMouse
LotC-7
  4
reviewed by:
Verified Customer
IHC-Fr Other 08/31/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedsciatic nerve
SpeciesOther
Lotc1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Stathmin-2/STMN2 Antibody (NBP1-49461) (0)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Kevin Thorburn
11/29/2018
Application: IHC-Fr
Species: Mouse

 
Verified Customer
08/31/2016
Application: IHC-Fr
Species: Other

Bioinformatics

Gene Symbol STMN2