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SPIC Antibody (PCRP-SPIC-2C5)

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Immunocytochemistry/ Immunofluorescence: SPIC Antibody (PCRP-SPIC-2C5) [NBP3-07570] - Immunofluorescent Analysis of PFA-fixed HeLa cells. SPIC Mouse Monoclonal Antibody (PCRP-SPIC-2C5) followed by IgG-CF488 (green), ...read more
Flow Cytometry: SPIC Antibody (PCRP-SPIC-2C5) [NBP3-07570] - Flow Cytometric Analysis of PFA-fixed HeLa cells. SPIC Mouse Monoclonal Antibody (PCRP-SPIC-2C5) followed by goat anti- Mouse IgG-CF488 (blue); unstained ...read more
Protein Array: SPIC Antibody (PCRP-SPIC-2C5) [NBP3-07570] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SPIC Mouse Monoclonal Antibody (PCRP-SPIC-2C5).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, MA
Clone
PCRP-SPIC-2C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

SPIC Antibody (PCRP-SPIC-2C5) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08334)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Recombinant full-length human SPIC protein (Uniprot: Q8N5J4)
Localization
Nucleus
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SPIC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for SPIC Antibody (PCRP-SPIC-2C5)

  • MGC40611
  • Spi-C transcription factor (Spi-1/PU.1 related)
  • SPI-C
  • transcription factor Spi-C

Background

Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis. Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene expression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for SPIC Antibody (NBP3-07570) (0)

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol SPIC
Uniprot