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SP140 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517] - Staining in human spleen and cerebral cortex tissues using anti-SP140 antibody. Corresponding SP140 RNA-seq data are presented ...read more
Immunocytochemistry/ Immunofluorescence: SP140 Antibody [NBP2-49517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & mitochondria.
Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517] - Staining of human spleen shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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SP140 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LSLLPGEGEEGSDDCSEMCDGEERQEASSSLARRGSVSSELENHPMNEEGESEELASSLLYDNVPGAEQSA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SP140
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SP140 Recombinant Protein Antigen (NBP2-49517PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SP140 Antibody

  • Lymphoid-restricted homolog of Sp100
  • lymphoid-specific SP100 homolog
  • LYSP100
  • LYSP100-A
  • LYSP100-B
  • MGC126440
  • Nuclear autoantigen Sp-140
  • nuclear body protein SP140
  • SP140 nuclear body protein
  • Speckled 140 kDa

Background

SP140 is a component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SP140