![Western Blot: SP1 Antibody (5I3A8) [NBP3-15837] - Western blot analysis of extracts of various cell lines, using SP1 antibody (NBP3-15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Western-Blot-NBP3-15837-img0005.jpg "Western Blot: SP1 Antibody (5I3A8) [NBP3-15837] - Western blot analysis of extracts of various cell lines, using SP1 antibody (NBP3-15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, ChIP |
| Clone | 5I3A8 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human SP1 (P08047). TTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTR |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SP1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.05% BSA, 50% glycerol, pH7.3 |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunocytochemistry__Immunofluorescence_21634.jpg)
![Immunohistochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunohistochemistry_9938.jpg)
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SP1 |
![Immunocytochemistry/Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837] - Immunofluorescence analysis of NIH-3T3 cells using SP1 antibody (NBP3-15837). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Immunocytochemistry-Immunofluorescence-NBP3-15837-img0004.jpg "Immunocytochemistry/Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837] - Immunofluorescence analysis of NIH-3T3 cells using SP1 antibody (NBP3-15837). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: SP1 Antibody (5I3A8) [NBP3-15837] - Immunohistochemistry of paraffin-embedded mouse kidney using SP1 antibody (NBP3-15837) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Immunohistochemistry-Paraffin-NBP3-15837-img0002.jpg "Immunohistochemistry-Paraffin: SP1 Antibody (5I3A8) [NBP3-15837] - Immunohistochemistry of paraffin-embedded mouse kidney using SP1 antibody (NBP3-15837) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [NBP3-15837] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody (NBP3-15837) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Chromatin-Immunoprecipitation-NBP3-15837-img0006.jpg "Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [NBP3-15837] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody (NBP3-15837) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.")
![Immunocytochemistry/Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837] - Immunofluorescence analysis of C6 cells using SP1 antibody (NBP3-15837). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Immunocytochemistry-Immunofluorescence-NBP3-15837-img0003.jpg "Immunocytochemistry/Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837] - Immunofluorescence analysis of C6 cells using SP1 antibody (NBP3-15837). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: SP1 Antibody (5I3A8) [NBP3-15837] - Immunohistochemistry of paraffin-embedded rat spleen using SP1 antibody (NBP3-15837) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Immunohistochemistry-Paraffin-NBP3-15837-img0001.jpg "Immunohistochemistry-Paraffin: SP1 Antibody (5I3A8) [NBP3-15837] - Immunohistochemistry of paraffin-embedded rat spleen using SP1 antibody (NBP3-15837) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
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![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")