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SORBS1 Antibody

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Immunohistochemistry: SORBS1 Antibody [NBP1-06079] - (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry: SORBS1 Antibody [NBP1-06079] - (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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SORBS1 Antibody Summary

Immunogen
Peptide with sequence C-SERNDWEPPDKKVD corresponding to internal region according to NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1, NP_001030129.1.
Epitope
C-SERNDWEPPDKKVD
Specificity
This antibody is expected to recognise all reported isoforms (NP_006425.2 and NP_056200.1 and NP_001030126.1 and NP_001030127.1 and NP_001030128.1 and NP_079267.1 and NP_001030129.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SORBS1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 65 kDa band in human adipose lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6 kDa band according to NP_006425.2 and higher sizes for other isoforms. The 65 kDa band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-06079.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SORBS1 Antibody

  • c-Cbl associated protein
  • c-Cbl-associated protein
  • DKFZp451C066
  • DKFZp586P1422
  • Fas-ligand associated factor 2
  • FLAF2
  • KIAA0894
  • KIAA1296CAPSH3D5SH3P12FLJ12406
  • ponsin
  • R85FL
  • SH3 domain protein 5
  • SH3-domain protein 5 (ponsin)
  • sh3p12
  • SORB1
  • sorbin and SH3 domain containing 1
  • sorbin and SH3 domain-containing protein 1

Background

SORBS1 plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SORBS1 Antibody (NBP1-06079)(1)

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Bioinformatics

Gene Symbol SORBS1
Entrez
Uniprot