Home » SOD3/EC-SOD » SOD3/EC-SOD Antibodies » SOD3/EC-SOD Antibody (713707) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

SOD3/EC-SOD Antibody (713707) [Unconjugated]

 (1 Publication)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human SOD3/EC-SOD Monoclonal Antibody (Catalog # MAB34201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOD3/EC-SOD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human SOD3/EC-SOD Monoclonal Antibody (Catalog # MAB34201) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Simple Western shows lysates of mouse aorta, loaded at 0.5 mg/ml. A specific band was detected for SOD3/EC‑SOD at approximately 41 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human SOD3/EC‑SOD ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clone
713707
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB34201-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

SOD3/EC-SOD Antibody (713707) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOD3/EC-SOD
Trp19-Ala240
Accession # P08294
Specificity
Detects human SOD3/EC-SOD in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SOD2, recombinant mouse (rm) SOD1, or rmSOD3 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SOD3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using MAB34201.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOD3/EC-SOD Antibody (713707) [Unconjugated]

  • ECSOD
  • EC-SOD
  • EC-SODEC 1.15.1.1
  • extracellular superoxide dismutase [Cu-Zn]
  • MGC20077
  • SOD3
  • superoxide dismutase 3, extracellular

Background

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring, but harmful, superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutase 3 (SOD3) also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. Human SOD3 shares ~64% sequence homology with mouse and rat SOD3. Like SOD1, SOD3 binds one Cu2+ and Zn2+ ions per subunit but differs in sequence and tissue distribution. SOD3 is a secretory protein and is synthesized with a putative 18-amino acid signal peptide preceding the 222 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP‑dependent oxidative system of neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

SOD1/Cu-Zn SOD
Antibody
NBP2-24915
Western Blot SOD1/Cu-Zn SOD Antibody
Western Blot SOD1/Cu-Zn SOD Antibody
Immunohistochemistry-Paraffin SOD1/Cu-Zn SOD Antibody
(3 Reviews)
(20 Publications)
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
SOD2/Mn-SOD
Antibody
NB100-1992
Immunohistochemistry SOD2/Mn-SOD Antibody
Immunocytochemistry/ Immunofluorescence SOD2/Mn-SOD Antibody
Immunocytochemistry/ Immunofluorescence SOD2/Mn-SOD Antibody
(42 Publications)
Species: Bv, Ca, Ch, Dr, Gt, Gp, Ha, Hu, Pm, Mu, Po, Rb, Rt, Sh, Sq, Xp
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Catalase Antibody
[Unconjugated]
AF3398
Western Blot Catalase Antibody [Unconjugated]
Simple Western Catalase Antibody [Unconjugated]
Knockout Validated Catalase Antibody [Unconjugated]
(3 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(808 Publications)
Species: Hu
Applications: BA
iNOS Antibody -
BSA Free
NB300-605
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
(3 Reviews)
(130 Publications)
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
CRAT Antibody
NBP1-86616
Western Blot CRAT Antibody
Western Blot CRAT Antibody
Immunohistochemistry-Paraffin CRAT Antibody
(2 Publications)
Species: Hu
Applications: IHC, IHC-P, WB
eNOS Antibody -
BSA Free
NB300-500
Western Blot eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
(3 Reviews)
(28 Publications)
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr, IHC-P, WB
GLYAT Antibody
NBP2-58917
Western Blot GLYAT Antibody
Immunohistochemistry-Paraffin GLYAT Antibody
Immunohistochemistry-Paraffin GLYAT Antibody
Species: Hu
Applications: IHC, IHC-P, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(639 Publications)
Species: Mu
Applications: ELISA
Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
(3 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
Glutathione
Peroxidase 1/GPX1 Ant ...
AF3798
Western Blot Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]
Simple Western Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]
Immunohistochemistry Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]
(29 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
Glutaminyl-peptide
Cyclotransferase/QPCT ...
NBP1-81838
Western Blot Glutaminyl-peptide Cyclotransferase/QPCT Antibody
Immunohistochemistry Glutaminyl-peptide Cyclotransferase/QPCT Antibody
Immunohistochemistry-Paraffin Glutaminyl-peptide Cyclotransferase/QPCT Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(676 Publications)
Species: Hu
Applications: ELISA
Nanos3 Antibody -
BSA Free
NBP1-76919
Immunocytochemistry/ Immunofluorescence Nanos3 Antibody - BSA Free
Western Blot Nanos3 Antibody - BSA Free
Immunohistochemistry-Paraffin Nanos3 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Xanthine Oxidase
Antibody (0M8E7)
NBP3-16735
Immunohistochemistry-Paraffin Xanthine Oxidase Antibody (0M8E7)
Immunohistochemistry-Paraffin Xanthine Oxidase Antibody (0M8E7)
Immunohistochemistry-Paraffin Xanthine Oxidase Antibody (0M8E7)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
HO-1/HMOX1/HSP32
Antibody (HO-1-1)
NBP1-97507
Flow Cytometry HO-1/HMOX1/HSP32 Antibody (HO-1-1)
Immunohistochemistry-Paraffin HO-1/HMOX1/HSP32 Antibody (HO-1-1)
Western Blot HO-1/HMOX1/HSP32 Antibody (HO-1-1)
(1 Review)
(20 Publications)
Species: Bv, Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Cytochrome b245
alpha Antibody (CS9)
NBP1-40974
Flow Cytometry Cytochrome b245 alpha Antibody (CS9)
Flow Cytometry Cytochrome b245 alpha Antibody (CS9)
(1 Publication)
Species: Hu
Applications: ELISA, Flow, IHC, IHC-Fr, IP, WB
CYBB/NOX2 Antibody
- BSA Free
NBP2-41291
Immunohistochemistry-Paraffin CYBB/NOX2 Antibody - BSA Free
Immunohistochemistry-Paraffin CYBB/NOX2 Antibody - BSA Free
Immunohistochemistry CYBB/NOX2 Antibody - BSA Free
(1 Review)
(7 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB

Publications for SOD3/EC-SOD Antibody (MAB34201)(1)


Reviews for SOD3/EC-SOD Antibody (MAB34201) (0)

There are no reviews for SOD3/EC-SOD Antibody (MAB34201). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOD3/EC-SOD Antibody (MAB34201) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SOD3/EC-SOD Products

Blogs on SOD3/EC-SOD

There are no specific blogs for SOD3/EC-SOD, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SOD3/EC-SOD Antibody (713707) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol SOD3
Uniprot