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SOCS-3 Antibody (SO1) - BSA Free

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Western Blot: SOCS3 Antibody (SO1) [NB600-1075] - Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of HeLa human cervix carcinoma cell line. Lane 1: immunostaining with Isotype mouse IgG2b control (PLRV219) Lane 2: immunostaining with anti-SOCS3 (SO1).
Western Blot: SOCS3 Antibody (SO1) [NB600-1075] - Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of HeLa human cervix carcinoma cell line. Lane 1: immunostaining with Isotype mouse IgG2b ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clone
SO1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-1075AF350
Alexa Fluor 405
NB600-1075AF405
Alexa Fluor 488
NB600-1075AF488
Alexa Fluor 532
NB600-1075AF532
Alexa Fluor 594
NB600-1075AF594
Alexa Fluor 647
NB600-1075AF647
Alexa Fluor 700
NB600-1075AF700
Alexa Fluor 750
NB600-1075AF750
Biotin
NB600-1075B
DyLight 350
NB600-1075UV
DyLight 405
NB600-1075V
DyLight 488
NB600-1075G
DyLight 550
NB600-1075R
DyLight 594
NB600-1075DL594
DyLight 650
NB600-1075C
DyLight 680
NB600-1075FR
DyLight 755
NB600-1075IR
HRP
NB600-1075H
Janelia Fluor 549
NB600-1075JF549
Janelia Fluor 646
NB600-1075JF646
mFluor Violet 450 SE
NB600-1075MFV450
mFluor Violet 500 SE
NB600-1075MFV500
mFluor Violet 610 SE
NB600-1075MFV610
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SOCS-3 Antibody (SO1) - BSA Free Summary

Immunogen
Full-length SOCS3 protein.
Specificity
The antibody SO1 reacts with SOCS3, an intracellular cytokine signaling inhibitor.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SOCS3
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.
Publications
Read Publication using
NB600-1075 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24142708).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for SOCS-3 Antibody (SO1) - BSA Free

  • ATOD4
  • CIS3
  • CIS3CIS-3
  • Cish3
  • cytokine-induced SH2 protein 3
  • Cytokine-inducible SH2 protein 3
  • SOCS3
  • SOCS-3
  • SOCS-3STAT-induced STAT inhibitor 3
  • SSI-3
  • SSI-3ATOD4
  • SSI3MGC71791
  • suppressor of cytokine signaling 3

Background

SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SOCS-3 Antibody
(SO1) - BSA Free
NB600-1075
SOCS-3 Antibody (SO1) - BSA Free
Species: Hu, Rt
Applications: WB

Publications for SOCS-3 Antibody (NB600-1075)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol SOCS3
Entrez