Western Blot: SOCS-1 Antibody [NB100-56637] - Analysis of SOCS-1 using NB100-56637 at 1:1000 dilution against 15 ug/lane of Ramos (A) and Raw (B) cell lysate.
Product Details
Summary
Reactivity
Hu, MuSpecies Glossary
Applications
WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
23.55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 1 using NB100-56637 in the following application:
This should cross-react with rat SOCS1, based on amino acid sequence.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified
Alternate Names for SOCS-1 Antibody
CIS1
Cish1
JAB
JABTec-interacting protein 3
JAK binding protein
JAK-binding protein
SOCS1
SOCS-1
SOCS-1CIS1
SSI-1
SSI-1CISH1
SSI1cytokine-inducible SH2 protein 1
STAT induced SH3 protein 1
STAT-induced STAT inhibitor 1
suppressor of cytokine signaling 1
TIP3
TIP-3
TIP3Cish1
Background
The suppressor of cytokine signaling (SOCS) family of proteins (SOCS1-SOCS7 and CIS) inhibit cytokine production by a classical negative feedback mechanism. These proteins are upregulated in response to cytokine stimulation followed by inhibition of cytokine-inducing signal pathway. Role of each members of the family has been determined by gene knockout experiments (1). SOCS1 also known as JAB and (STAT)-induced STAT inhibitor 1 (SSI-1) is an SH2-domain-containing protein that is induced by and negatively regulates signaling by a number of cytokines including interleukin-4 (IL-4), IL-6, interferon (IFN)-gamma, prolactin, growth hormone, and erythropoietin (1). SOCS1/JAB is rapidly induced by LPS and negatively regulates LPS signaling (2). SOCS1 (-/-) mice are very sensitive to LPS. These mice die prematurely, probably due to uncontrolled IFN-g signaling (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Brain,EBV-transformed lymphoblastoid B-cell lines from Healthy control
Species
Human
Lot
111302C-04
Comments
Comments
***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Samples : LCL : EBV-transformed lymphoblastoid B-cell lines from Healthy control (Ctle) : Concentrations : 0,5 – 1 – 2 mg/mL (total protein concentration) SOCS1 antibody dilution : 1/10 – 1/50 – 1/100
Product General Protocols
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