![Western Blot: SLC28A3 Antibody [NBP2-29615] - Western blot analysis of SLC28A3 in A. PC3 cell lysate B. PC3 lysate with blocking peptide.](http://images.novusbio.com/fullsize/SLC28A3-Antibody-Western-Blot-NBP2-29615-img0001.jpg "Western Blot: SLC28A3 Antibody [NBP2-29615] - Western blot analysis of SLC28A3 in A. PC3 cell lysate B. PC3 lysate with blocking peptide.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide made to an internal portion of the human SLC28A3 protein (between residues 30-100) [NCBI NP_071410.1] |
| Localization | Multipass transmembrane protein with Cytoplasmic, Helical and Extracellular domains |
| Predicted Species | Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC28A3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | SLC28A3 is a multipass membrane protein and its canonical form (Isoform 1) is 691AA long with predicted molecular weight of 76.9 kDa. This SLC28A3 antibody is not expected to detect Isoform 2 of SLC28A3, and in our testing with WB assay, this antibody detected the target band (~75kDa) with one additional/uncharacterized band (~17 kda). Because the immunogen sequence of this SLC28A3 antibody falls within the first cytoplasmic domain of SLC28A3 (see Uniprot# Q9HAS3; aa1-100, the largest cytoplasmic domain of this protein), the expected staining pattern with this antibody would be cytoplasmic in immunostaining assays. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: SLC28A3 Antibody [NBP2-29615] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human pancreas using SLC28A3 antibody at 1:200 dilution. The antibody generated an expected cytoplasmic staining and the expression was mainly seen in Islet of Langerhans while the pancreatic acinar cells showed very weak staining. Inside the Islet of Langerhans, the alpha and beta cells showed moderate to high staining while the delta cells depicted very intense staining for SLC28A3 protein.](http://images.novusbio.com/fullsize/SLC28A3-Antibody-Immunohistochemistry-Paraffin-NBP2-29615-img0002.jpg "Immunohistochemistry-Paraffin: SLC28A3 Antibody [NBP2-29615] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human pancreas using SLC28A3 antibody at 1:200 dilution. The antibody generated an expected cytoplasmic staining and the expression was mainly seen in Islet of Langerhans while the pancreatic acinar cells showed very weak staining. Inside the Islet of Langerhans, the alpha and beta cells showed moderate to high staining while the delta cells depicted very intense staining for SLC28A3 protein.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")