GLI-2 Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Mouse GLI-2 is a 185 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least two additional isoforms that have amino acid substitutions/truncations in the region corresponding to amino acid residues 192‑350 of isoform 1 have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, mouse GLI-2 shares 91% and 97% amino acid sequence homology with that of human and rat GLI-2, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Xenopus.

We have publications tested in 5 applications: ICC, IHC-P, Immunocytochemistry, Immunoprecipitation, Western Blot.

Showing Publications 1 - 10 of 44. Show All 44 Publications. Collapse Publications.

Publications using AF3635	Applications	Species
U?pie?ski, T;Niewiadomski, P; The Proteasome and Cul3-Dependent Protein Ubiquitination Is Required for Gli Protein-Mediated Activation of Gene Expression in the Hedgehog Pathway Cells 2024-09-06 [PMID: 39273066] (Western Blot, Mouse)	Western Blot	Mouse
Baran, B;Derua, R;Janssens, V;Niewiadomski, P; PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathway Cellular signalling 2024-08-20 [PMID: 39173855] (Western Blot, Human)	Western Blot	Human
Shi, P;Tian, J;Mallinger, JC;Ling, D;Deleyrolle, LP;McIntyre, JC;Caspary, T;Breunig, JJ;Sarkisian, MR; Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary Cilia Cells 2023-09-26 [PMID: 37830570] (Immunocytochemistry, Mouse)	Immunocytochemistry	Mouse
Meropi Bagka, Hyeonyi Choi, Margaux Héritier, Hanna Schwaemmle, Quentin T. L. Pasquer, Simon M. G. Braun et al. Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog pathway inhibitor-1 Nature Communications 7/1/2023 [PMID: 37393376]
Fan Yang, Jezabel Rodriguez-Blanco, Jun Long, Marzena Swiderska-Syn, Daniel T. Wynn, Bin Li et al. A Druggable UHRF1/DNMT1/GLI Complex Regulates Sonic Hedgehog–Dependent Tumor Growth Molecular Cancer Research 11/3/2022 [PMID: 35925047]
J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell Reports, 2022-02-01;38(5):110312. 2022-02-01 [PMID: 35108539] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
Sun-Hee Hwang, Bandarigoda N Somatilaka, Kevin White, Saikat Mukhopadhyay Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner eLife 8/4/2021 [PMID: 34346313]
Hualing Peng, Jingyi Zhang, Amanda Ya, Winston Ma, Sammy Villa, Shahar Sukenik et al. Myomegalin regulates Hedgehog pathway by controlling PDE4D at the centrosome Molecular Biology of the Cell 9/1/2021 [PMID: 34260267]
A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy Cancers, 2021-04-15;13(8):. 2021-04-15 [PMID: 33921042] (Western Blot, Mouse)	Western Blot	Mouse
CW Wasson, RL Ross, R Wells, C Corinaldes, IC Georgiou, NA Riobo-Del, F Del Galdo Long non-coding RNA HOTAIR induces GLI2 expression through Notch signalling in systemic sclerosis dermal fibroblasts Arthritis Research & Therapy, 2020-12-10;22(1):286. 12/10/2020 [PMID: 33303026]
JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells Science Advances, 2020-09-16;6(38):. 2020-09-16 [PMID: 32938678] (ICC, Immunoprecipitation, Western Blot, Mouse)	ICC, Immunoprecipitation, Western Blot	Mouse
Saishu Yoshida, Katsuhiko Aoki, Ken Fujiwara, Takashi Nakakura, Akira Kawamura, Kohji Yamada et al. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis eLife 8/6/2020 [PMID: 32758357]
Bandarigoda Nipunika Somatilaka, Sun-Hee Hwang, Vivek Reddy Palicharla, Kevin Andrew White, Hemant Badgandi, John Michael Shelton et al. Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling Developmental Cell 9/1/2020 [PMID: 32702291]
Marisa E. Hom, Alison E. Ondrus, Tomoyo Sakata‐Kato, Paul G. Rack, James K. Chen Bicyclic Imidazolium Inhibitors of Gli Transcription Factor Activity ChemMedChem 6/17/2020 [PMID: 32268014]
Sandii Constable, Alyssa B. Long, Katharine A. Floyd, Stéphane Schurmans, Tamara Caspary The ciliary phosphatidylinositol phosphatase Inpp5e plays positive and negative regulatory roles in Shh signaling Development 1/1/2020 [PMID: 31964774]
L Lospinoso, D Quaglio, I Basili, F Ghirga, F Bufalieri, M Caimano, S Balducci, M Moretti, I Romeo, E Loricchio, M Maroder, B Botta, M Mori, P Infante, L Di Marcotu A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth Cancers (Basel), 2019-10-09;11(10):. 2019-10-09 [PMID: 31601026] (Western Blot, Mouse)	Western Blot	Mouse
A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling Sci Rep, 2019-09-25;9(1):13896. 2019-09-25 [PMID: 31554934] (ICC, Western Blot, Mouse)	ICC, Western Blot	Mouse
Francesca Bufalieri, Paola Infante, Flavia Bernardi, Miriam Caimano, Paolo Romania, Marta Moretti et al. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of beta TrCP Nature Communications 7/24/2019 [PMID: 31341163]
P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning Cell Rep, 2019-07-09;28(2):541-553.e4. 2019-07-09 [PMID: 31291587] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
Yuhong Han, Bing Wang, Yong Suk Cho, Jian Zhu, Jiang Wu, Yongbin Chen et al. Phosphorylation of Ci/Gli by Fused Family Kinases Promotes Hedgehog Signaling Developmental Cell 9/1/2019 [PMID: 31279575]
Alexandre Wojcinski, Morgane Morabito, Andrew K. Lawton, Daniel N. Stephen, Alexandra L. Joyner Genetic deletion of genes in the cerebellar rhombic lip lineage can stimulate compensation through adaptive reprogramming of ventricular zone-derived progenitors Neural Development 12/1/2019 [PMID: 30764875]
Teresa Purzner, James Purzner, Taylor Buckstaff, Giorgio Cozza, Sharareh Gholamin, Jessica M. Rusert et al. Developmental phosphoproteomics identifies the kinase CK2 as a driver of Hedgehog signaling and a therapeutic target in medulloblastoma Science Signaling 9/13/2018 [PMID: 30206138]
A Ooki, W Dinalankar, L Marchionni, JJ Tsay, C Goparaju, Z Maleki, WN Rom, HI Pass, MO Hoque Epigenetically regulated PAX6 drives cancer cells toward a stem-like state via GLI-SOX2 signaling axis in lung adenocarcinoma Oncogene, 2018-07-06;0(0):. 7/6/2018 [PMID: 29980786]
P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis Nat Commun, 2018-03-07;9(1):976. 2018-03-07 [PMID: 29515120] (Western Blot, Mouse)	Western Blot	Mouse
T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms Cell. Mol. Life Sci., 2018-02-02;0(0):. 2018-02-02 [PMID: 29396625] (Western Blot, Mouse)	Western Blot	Mouse
VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling Proc. Natl. Acad. Sci. U.S.A., 2017-11-20;0(0):. 2017-11-20 [PMID: 29158396] (IHC-P, Mouse)	IHC-P	Mouse
Jesse D. Riordan, Charlotte R. Feddersen, Barbara R. Tschida, Pauline J. Beckmann, Vincent W. Keng, Michael A. Linden et al. Chronic liver injury alters driver mutation profiles in hepatocellular carcinoma in mice Hepatology 3/1/2018 [PMID: 28961327]
J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development J. Biol. Chem., 2017-08-09;0(0):. 2017-08-09 [PMID: 28794157] (Western Blot, Mouse)	Western Blot	Mouse
Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system PLoS Biol., 2017-08-04;15(8):e2002063. 2017-08-04 [PMID: 28777795] (Western Blot, Mouse)	Western Blot	Mouse
Roberta Quaranta, Maria Pelullo, Sabrina Zema, Francesca Nardozza, Saula Checquolo, Dieter Matthias Lauer et al. Maml1 acts cooperatively with Gli proteins to regulate sonic hedgehog signaling pathway Cell Death & Disease 7/20/2017 [PMID: 28726779]
Maria A. Hoelzl, Karin Heby-Henricson, Marco Gerling, José M. Dias, Raoul V. Kuiper, Cornelius Trünkle et al. Differential requirement of SUFU in tissue development discovered in a hypomorphic mouse model Developmental Biology 9/1/2017 [PMID: 28688895]
H Cai, A Liu Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord Dev. Biol., 2017-04-12;0(0):. 2017-04-12 [PMID: 28412462] (Western Blot, Mouse)	Western Blot	Mouse
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):. 2016-12-05 [PMID: 27930311] (Western Blot, Human)	Western Blot	Human
Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-?/?1-Independent PLoS ONE, 2016-08-31;11(8):e0162033. 2016-08-31 [PMID: 27579771] (Western Blot, Mouse)	Western Blot	Mouse
David S Lorberbaum, Andrea I Ramos, Kevin A Peterson, Brandon S Carpenter, David S Parker, Sandip De et al. An ancient yet flexible cis-regulatory architecture allows localized Hedgehog tuning by patched/Ptch1 eLife 5/5/2016 [PMID: 27146892]
Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang S, Rennke H, Waikar S, Humphreys B Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis. J Clin Invest, 2015-07-20;125(8):2935-51. 2015-07-20 [PMID: 26193634] (Western Blot, Mouse)	Western Blot	Mouse
S Pandolfi, V Montagnani, A Lapucci, B Stecca HEDGEHOG/GLI-E2F1 axis modulates iASPP expression and function and regulates melanoma cell growth Cell Death & Differentiation 12/1/2015 [PMID: 26024388]
Belgacem Y, Borodinsky L Inversion of Sonic hedgehog action on its canonical pathway by electrical activity. Proc Natl Acad Sci U S A, 2015-03-17;112(13):4140-5. 2015-03-17 [PMID: 25829542] (IHC-P, Western Blot, Xenopus)	IHC-P, Western Blot	Xenopus
Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators. J Clin Invest, 2014-04-01;124(5):2059-70. 2014-04-01 [PMID: 24691443] (Western Blot, Mouse)	Western Blot	Mouse
Alla Piirsoo, Lagle Kasak, Mari-Liis Kauts, Mart Loog, Kairit Tints, Piia Uusen et al. Protein kinase inhibitor SU6668 attenuates positive regulation of Gli proteins in cancer and multipotent progenitor cells Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 4/1/2014 [PMID: 24418624]
Donnelly J, Engevik A, Engevik M, Schumacher M, Xiao C, Yang L, Worrell R, Zavros Y Gastritis promotes an activated bone marrow-derived mesenchymal stem cell with a phenotype reminiscent of a cancer-promoting cell. Dig Dis Sci, 2013-11-08;59(3):569-82. 11/8/2013 [PMID: 24202649]
Regard JB, Malhotra D, Gvozdenovic-Jeremic J et al. Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification. Nat Med. 9/29/2013 [PMID: 24076664]
Liu HX, Ermilov A, Grachtchouk M et al. Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance. Dev Biol 8/2/2013 [PMID: 23916850]
Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins. Genes Dev., 2010-04-01;24(7):670-82. 2010-04-01 [PMID: 20360384] (Immunoprecipitation, Western Blot, Mouse)	Immunoprecipitation, Western Blot	Mouse
Show All 44 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Mouse	11/13/2015	View Summary Application Western Blot Sample Tested NIH-3T3 cells Species Mouse
Enlarge	4 reviewed by: Verified Customer	IF	Mouse	11/13/2015	View Summary Application Immunofluorescence Sample Tested NIH-3T3 cells Species Mouse

Array AF3635

• GLI-2 Antibodies
• GLI-2 Lysate
• GLI-2 Proteins